In the present study, a proteomic technology combining one-dimensional gel electrophoresis (1DE) with subsequent mass spectrometry (MALDI-TOF-PMF) has been successfully applied for revelation of changes in the protein profile of zebrafish (Danio rerio) 52 hpf embryos. Prior to 1DE separation of zebrafish embryonic proteins, the procedure for obtaining embryos homogenate was optimized by ultrasonic treatment. A total of 84 proteins, including 15 vitellogenins, were identified. It was shown that growing of zebrafish embryos in the medium with doxorubicin (DOX) stimulated Caspase-3 induction and promoted the disappearance of cardiac troponins, both these findings being consistent with literature data on doxorubicin-induced cardiotoxicity. The 1DE-based proteomic mapping approach proposed herein enabled not only to identify proteins but also to register those changes in embryos' proteomic profile that were caused by doxorubicin.
Ito H., Miller S.C., Billingham M.E., Akimoto H., Torti S.V., Wade R., Gahlmann R., Lyons G., Kedes L., Torti F.M. (1990) Proc. Natl. Acad. Sci. USA, 87(11), 4275-4279. . CrossRef Scholar google search
Panaretakis T., Laane E., Pokrovskaja K., Bjorklund A.C., Moustakas A., Zhivotovsky B., Heyman M., Shoshan M.C., Grander D. (2005) Mol. Biol. Cell., 8, 3821-3831. . CrossRef Scholar google search