One-dimensional proteomic mapping of embryos danio rerio

   
Kisrieva J.S.1, Petushkova N.A.1 , Chernobrovkin A.S.1, Larina O.B.1, Trifonova O.P.2, Kuznetsova G.P.1, Samenkova N.F.1, Kashirceva V.N.1, Belayeva N.F.1, Karuzina I.I.1, Lisitsa A.V.1

1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
2. State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20115706593      UDK: 578.064      PubMed Id: 22359915
Year: 2011  Volume: 57  Issue: 6  Pages: 593-603
In the present study, a proteomic technology combining one-dimensional gel electrophoresis (1DE) with subsequent mass spectrometry (MALDI-TOF-PMF) has been successfully applied for revelation of changes in the protein profile of zebrafish (Danio rerio) 52 hpf embryos. Prior to 1DE separation of zebrafish embryonic proteins, the procedure for obtaining embryos homogenate was optimized by ultrasonic treatment. A total of 84 proteins, including 15 vitellogenins, were identified. It was shown that growing of zebrafish embryos in the medium with doxorubicin (DOX) stimulated Caspase-3 induction and promoted the disappearance of cardiac troponins, both these findings being consistent with literature data on doxorubicin-induced cardiotoxicity. The 1DE-based proteomic mapping approach proposed herein enabled not only to identify proteins but also to register those changes in embryos' proteomic profile that were caused by doxorubicin.
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Kisrieva J.S., Petushkova N.A., Chernobrovkin A.S., Larina O.B., Trifonova O.P., Kuznetsova G.P., Samenkova N.F., Kashirceva V.N., Belayeva N.F., Karuzina I.I., Lisitsa A.V. (2011) Biomeditsinskaya khimiya, 57(6), 593-603.
This paper is also available as the English translation:10.1134/S1990750812010040
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