Specificity of molecular recognition in oligomerization of bacterial L-asparaginases

Mezentsev Yu.V.1 , Molnar A.A.1, Sokolov N.N.1, Lisitsina V.B.2, Ivanov A.S.1, Archakov A.I.1, Archakov M.A.2

1. Russian Academy of Medical Science, Institute of Biomedical Chemistry, RAMS
2. Russian State Medical University
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20125801050      PubMed Id: 22642152
Year: 2012  Volume: 58  Issue: 1  Pages: 50-64
Bacterial L-asparaginases, which are widely used in the antitumor therapy, act only as homotetramers, because their active sites are located at the interface between the subunits of the enzyme. Since salt bridges substantially stabilize L-asparaginase tetramers, we have supposed that oligomerization of bacterial L-asparaginase is a high-avidity process. This assumption was proved by bioinformatic and biosensoric methods. It was shown, that a stable tetrameric complex can be formed only by the subunits of the same L-asparaginase. Using two mutants of L-asparaginase Helicobacter pylori it was shown that specificity of molecular recognition is significantly reduced even by single point mutation at the interface of high-homologous closely-related subunits.
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Keywords: specificity, molecular recognition, oligomerization, L-asparaginase, computer modeling, optical biosensor, surface plasmon resonance

Mezentsev, Yu. V., Molnar, A. A., Sokolov, N. N., Lisitsina, V. B., Ivanov, A. S., Archakov, A. I., Archakov, M. A. (2012). Specificity of molecular recognition in oligomerization of bacterial L-asparaginases. Biomeditsinskaya Khimiya, 58(1), 50-64.
This paper is also available as the English translation: 10.1134/S1990750811020107
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