Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence

   
Li K.1, Nu L.Z.1, Khe K.L.1, Song K.H.1

1. Northwest University (Sian, China)
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20125801088      PubMed Id: 22642155
Year: 2012  Volume: 58  Issue: 1  Pages: 88-94
A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng⋅ml-1 with the detection limit of 0.02 ng⋅ml-1 (3σ). At a flow rate of 2.0 ml⋅min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.
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Keywords: phenobarbital, chemiluminescence, luminol
Citation:

Li, K., Nu, L. Z., Khe, K. L., Song, K. H. (2012). Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence. Biomeditsinskaya khimiya, 58(1), 88-94.
This paper is also available as the English translation: 10.1134/S1990750811020077
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