1. M.V. Lomonosov Moscow State Academy of Fine Chemical Technology, ANO Institute of Biomedical Problems, M.V. Lomonosov Moscow State University 2. ANO Institute of Biomedical Problems 3. ANO Institute of Biomedical Problems, Institute of Human Morphology, Russian Academy of Medical Sciences 4. M.V. Lomonosov Moscow State Academy of Fine Chemical Technology 5. ANO Institute of Biomedical Problems School of Biology, M.V. Lomonosov Moscow State University
Complete profiles of phospholipid and ceramide molecular species from erythrocyte lipid extracts of children without carbohydrate metabolism disorders, and children with type 1 diabetes were compared by means of high performance liquid chromatography/mass spectrometry. For the first time a statistically significant increase (p<0.05) of lysophosphatidylcholine content in two groups of diabetic children with different duration of the disease (less than one year and more than one year) was found. Statistically significant changes in other lipid classes were not observed. The dependence of the content of phosphatidylcholine and phosphatidylethanolamine molecular species containing arachidonic acid residue (20:4) on the duration of the disease was found. The observed shift in lipid metabolism suggests of phospholipase A2 and chronic inflammatory process at different stages of diabetes mellitus, in cells (erythrocytes), which aer not involved in the immune response.
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Keywords: mass spectrometry, lipidomics, lysophosphatidylcholine, arachidonic acid, erythrocytes, type 1 diabetes
Citation:
Mityanina V.A., Kuptzov V.N., Saveliev S.V., Shvets V.I., Selishcheva A.A. (2012) Erythrocyte lipid composition at different stages of type 1 diabetes in children. Biomeditsinskaya Khimiya, 58(1), 95-103.
Mityanina V.A. et al. Erythrocyte lipid composition at different stages of type 1 diabetes in children // Biomeditsinskaya Khimiya. - 2012. - V. 58. -N 1. - P. 95-103.
Mityanina V.A. et al., "Erythrocyte lipid composition at different stages of type 1 diabetes in children." Biomeditsinskaya Khimiya 58.1 (2012): 95-103.
Mityanina, V. A., Kuptzov, V. N., Saveliev, S. V., Shvets, V. I., Selishcheva, A. A. (2012). Erythrocyte lipid composition at different stages of type 1 diabetes in children. Biomeditsinskaya Khimiya, 58(1), 95-103.
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