1. Kazan State University 2. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of RAS 3. Interregional Clinical Diagnostic Center
The role of transition metal ions in atherogenesis is controversial; they can participate in the hydroxyl radical generation and catalyze the reactive oxygen species neutralization reaction as cofactors of antioxidant enzymes. Using EPR spectroscopy, we revealed that 70% of the samples of aorta with atherosclerotic lesions possessed superoxide dismutase activity, 100% of the samples initiated Fenton reaction and demonstrated the presence of manganese paramagnetic centers. The sodA gene encoding manganese-dependent bacterial superoxide dismutase was not found in the samples of atherosclerotic plaques by PCR using degenerate primers. The data obtained indicates the perspectives of manganese analysis as a marker element in the express diagnostics of atherosclerosis.
Download PDF:
Keywords: atherosclerosis, superoxide dismutase, manganese, sodA gene
Lozhkin A.P. et al. Manganese in atherogenesis: detection, origin, and role // Biomeditsinskaya Khimiya. - 2012. - V. 58. -N 3. - P. 291-299.
Lozhkin A.P. et al., "Manganese in atherogenesis: detection, origin, and role." Biomeditsinskaya Khimiya 58.3 (2012): 291-299.
Lozhkin, A. P., Biktagirov, T. B., Gorshkiv, O. V., Timonina, E. V., Mamin, G. V., Orlinskii, S. B., Silkin, N. I., Chernov, V. M., Khairullin, R. N., Salakhov, M. Kh., Ilinskaya, O. N., Abdul'yanov, V. A. (2012). Manganese in atherogenesis: detection, origin, and role. Biomeditsinskaya Khimiya, 58(3), 291-299.
Giacconi R., Muti E., Malavolta M., Cipriano C., Costarelli L., Bernardini G., Gasparini N., Mariani E., Saba V., Boccoli G., Mocchegiani E. (2007) Mol. Med., 13, 388-395. CrossRef Scholar google search
Ren M., Rajendran R., Ning P., Tan Kwong Huat B., Ong C.N., Watt F., Jenner A., Halliwell B. (2006) Free. Radic. Biol. Med., 41, 222-225. CrossRef Scholar google search
Ren M.Q., Rajendran R., Pan N., Huat B.T.K., Halliwell B., Watt, F. (2005) Nucl. Instr. Meth. Phys. Res., 231, 251-256. CrossRef Scholar google search
Watt F., Rajendran R., Ren M.Q., Tan B.K.H., Halliwell B. (2006) Nucl. Instr. Meth. Phys. Res., 249, 646-652. CrossRef Scholar google search
