1. Department of Biophysics, Belarusian State University 2. Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Research Institute of Physico-Chemical Medicine 3. Republican Science-Practical Center of Cardiology 4. Institute for Experimental Medicine of the Russian Academy of Medical Sciences 5. Research Institute of Physico-Chemical Medicine
Using previously developed spectro-photometrical method (Bioorg. Khim. 2009. V. 35. pp. 629-639), a significant increase of myeloperoxidase (MPO) activity was found in blood plasma of patients with type 2 diabetes mellitus (DM2) without of cardiovascular complications, as well as with ischemic heart disease (IHD). Plasma MPO concentration measured by an enzyme-linked immunosorbent assay was significantly higher only in blood plasma of patient with DM2 and IHD. A direct and significant correlation between MPO activity and MPO concentration was observed only in blood plasma samples from healthy donors. Increased MPO activity did not correlate with MPO concentration in blood plasma of patients with DM2 and DM2 with IHD. Taken together, these results highlight the necessity for studying of the MPO role in the development of pathological processes to determine both the amount of enzyme and its peroxidase activity in the blood. The proposed approach gives comprehensive information about the relationship between MPO activity and MPO concentration in patient blood. Since the high concentration of MPO is a diagnostically significant parameter in the prediction of endothelial dysfunction and cardiovascular disease development, the obtained results evidence the contribution of MPO-dependent reactions in cardiovascular complications associated with diabetes. MPO activity may serve as an additional diagnostic criterion for determination of risk of IHD in DM patients.
Gorudko I.V., Kostevich V.A., Sokolov A.V., Buko I.V., Konstantinova E.E., Tsapaeva N.L., Mironova E.V., Zakharova E.T., Vasilyev V.B., Cherenkevich S.N., Panasenko O.M. (2012) Increased myelopepoxidase activity is a risk factor for ishemic heart disease in patients with diabetes mellitus. Biomeditsinskaya Khimiya, 58(4), 475-484.
Gorudko I.V. et al. Increased myelopepoxidase activity is a risk factor for ishemic heart disease in patients with diabetes mellitus // Biomeditsinskaya Khimiya. - 2012. - V. 58. -N 4. - P. 475-484.
Gorudko I.V. et al., "Increased myelopepoxidase activity is a risk factor for ishemic heart disease in patients with diabetes mellitus." Biomeditsinskaya Khimiya 58.4 (2012): 475-484.
Gorudko, I. V., Kostevich, V. A., Sokolov, A. V., Buko, I. V., Konstantinova, E. E., Tsapaeva, N. L., Mironova, E. V., Zakharova, E. T., Vasilyev, V. B., Cherenkevich, S. N., Panasenko, O. M. (2012). Increased myelopepoxidase activity is a risk factor for ishemic heart disease in patients with diabetes mellitus. Biomeditsinskaya Khimiya, 58(4), 475-484.
Zhang C., Yang J., Jennings L.K. (2004) Diabetes, 53, 2950-2959. Scholar google search
Jacobi J., Sela S., Cohen H.I., Chezar J., Kristal B. (2006) Am. J. Physiol. Heart Circ. Physiol., 290, H2051-H2058. Scholar google search
Shurtz-Swirski R., Sela S., Herskovits A. T., Shasha S.M., Shapiro G., Nasser L., Kristal B. (2001) Diabetes Care, 24, 104-110. Scholar google search
Stegenga M.E., van der Crabben S.N., Blumer R.M.E., Levi M., Meijers J.C.M., Serlie M.J., Tanck M.W.T., Sauerwein H.P., van der Poll T. (2008) Blood, 112, 82-89. Scholar google search
Daugherty A., Dunn J.L., Rateri D.L., Heinecke J.W. (1994) J. Clin. Invest., 94, 437-444. Scholar google search
Stenvinkel P., Rodriguez-Ayala E., Massy Z.A., Qureshi A.R., Barany P., Fellstrom B., Heimburger O., Lindholm B., Alvestrand A. (2006) Clin. J. Am. Soc. Nephrol., 1, 281-287. Scholar google search
Boker T., Augustin A.J., Breipohl W., Spitznas M., Lutz J. (1994) Graefes Arch. Clin. Exp. Ophthalmol., 232, 652-656. CrossRef Scholar google search
Uchimura K., Nagasaka A., Hayashi R., Makino M., Nagata M., Kakizawa H., Kobayashi T., Fujiwara K., Kato T., Iwase K., Shinohara R., Kato K., Itoh M. (1999) J. Diabetes Complications, 13, 264-270. CrossRef Scholar google search
Heilman K., Zilmer M., Zilmer K., Lintrop M., Kampus P., Kals J., Tillmann V. (2009) Diabetes Res. Clin. Pract., 84, 168-173. CrossRef Scholar google search
Schindhelm R.K., Alssema M., Diamant M., Teerlink T., Dekker J.M., Kok A., Kostense P.J., Nijpels G., Heine R.J., Scheffer P.G. (2008) Metabolism, 57, 262-267. CrossRef Scholar google search
