Mass spectrometry detection of monomeric renalase in human urine

Fedchenko V.I.1, Buneeva O.A.1, Kopylov A.T.1, Kaloshin A.A.1, Axenova L.N.1, Zgoda V.G.1, Medvedev A.E.1

1. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
Section: Short Communication
DOI: 10.18097/PBMC20125805599      PubMed Id: 23289302
Year: 2012  Volume: 58  Issue: 5  Pages: 599-607
Renalase is a recently discovered secretory protein, which is suggested to play a role (which still remains elusive) in regulation of blood pressure. Earlier it was purified from urine of healthy volunteers by means of ammonium sulfate fractionation and subsequent affinity chromatography (Xu et al. (2005) J. Clin. Invest., 115, 1275). The resultant purified preparation of renalase contained 2 proteins with molecular masses of 35 and 67-75 kDa. The authors believed that the latter represents a dimerization (aggregation) product of the 35 kDa protein. In this study we have detected relanase in urinary samples of 2 of 6 volunteers only after immunoaffinity enrichment of urinary samples subjected to ammonium sulfate precipitation. Electrophoresis of the purified preparation also demonstrated the presence of 2 proteins with molecular masses of 35 and 66 kDa, respectively. Mass spectrometry analysis of these proteins identified 35 and 66 kDa proteins as renalase and serum albumin, respectively. Thus, our results do not support suggestion on formation of renalase dimers and they indicate that urinary renalase excretion significantly varies in humans.
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Keywords: renalase, urine, fractionation, immunoaffinity enrichment, mass spectrometry analysis

Fedchenko, V. I., Buneeva, O. A., Kopylov, A. T., Kaloshin, A. A., Axenova, L. N., Zgoda, V. G., Medvedev, A. E. (2012). Mass spectrometry detection of monomeric renalase in human urine. Biomeditsinskaya Khimiya, 58(5), 599-607.
This paper is also available as the English translation: 10.1134/s1990750812040038
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