Now it is absolutely clear, that the majority of proteins in living systems function due to interaction with each other in stable or dynamic proteins complexes. Therefore necessity of deeper studies of proteins functions causes expansion of protein-protein interaction research. In the present review the brief description and comparative estimation of experimental methods and protocols of protein interactomics, based on technology of molecular fishing on an optical chips and paramagnetic nanoparticles is given.
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Keywords: interactomics, protein-protein interaction, proteomics, molecular fishing, optical biosensor, magnetic particles, protein mass-spectrometry
Citation:
Ivanov A.S., Ershov P.V., Mezentsev Yu.V., Poverennaya E.V., Lisitsa A.V., Archakov A.I. (2013) Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles. Biomeditsinskaya Khimiya, 59(2), 171-182.
Ivanov A.S. et al. Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles // Biomeditsinskaya Khimiya. - 2013. - V. 59. -N 2. - P. 171-182.
Ivanov A.S. et al., "Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles." Biomeditsinskaya Khimiya 59.2 (2013): 171-182.
Ivanov, A. S., Ershov, P. V., Mezentsev, Yu. V., Poverennaya, E. V., Lisitsa, A. V., Archakov, A. I. (2013). Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles. Biomeditsinskaya Khimiya, 59(2), 171-182.
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