Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles

   
Ivanov A.S.1 , Ershov P.V.1, Mezentsev Yu.V.1, Poverennaya E.V.1, Lisitsa A.V.1, Archakov A.I.1

1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
Section: Review
DOI: 10.18097/pbmc20135902171      PubMed Id: 23789344
Year: 2013  Volume: 59  Issue: 2  Pages: 171-182
Now it is absolutely clear, that the majority of proteins in living systems function due to interaction with each other in stable or dynamic proteins complexes. Therefore necessity of deeper studies of proteins functions causes expansion of protein-protein interaction research. In the present review the brief description and comparative estimation of experimental methods and protocols of protein interactomics, based on technology of molecular fishing on an optical chips and paramagnetic nanoparticles is given.
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Keywords: interactomics, protein-protein interaction, proteomics, molecular fishing, optical biosensor, magnetic particles, protein mass-spectrometry
Citation:

Ivanov, A. S., Ershov, P. V., Mezentsev, Yu. V., Poverennaya, E. V., Lisitsa, A. V., Archakov, A. I. (2013). Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles. Biomeditsinskaya khimiya, 59(2), 171-182.
This paper is also available as the English translation: 10.1134/S1990750812020072
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