Recombinant intracellular Rhodospirillum rubrum L-asparaginase with low L-glutaminase activity and antiproliferative effect

Pokrovskaya M.V.1, Pokrovskiy V.S.1 , Aleksandrova S.S.1, Anisimova N.Yu.2, Andrianov R.M.3, Treschalina E.M.2, Ponomarev G.V.1, Sokolov N.N.1

1. Institute of Biomedical Chemistry of Russian Academy of Medical Sciences
2. N.N. Blokhin Cancer Research Center of Russian Academy of Medical Sciences
3. A.N. Bach Institute of biochemistry of Russian Academy of Sciences
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20135902192      UDK: 612.015.1577.151.6      PubMed Id: 23789346
Year: 2013  Volume: 59  Issue: 2  Pages: 192-208
The recombinant producer of Rhodospirillum rubrum L-asparaginase (RrA) was received and purification procedure of RrA was developed. It was shown that RrA has following biochemical and catalytic characteristics: K for L-asn 0,22 мM, pH optimum 9,2; temperature optimum 54°С; pI=5,1±0,3; L-gln activity seems to be low-to-negligible. К562, DU145 and MDA-MB-231 cellular lines displayed significant sensitivity towards the enzyme (IC50=1,80; 9,19 and 34,62 ME/ml, respectively. In comparison with L-asparaginases from E . coli II type (EcA) and Erwinia carotovora (EwA) cytotoxicity of RrA seems to be higher than EwA, but lower than EcA. 10-fold i.p. RrA administration (4000 ME/kg per day) in L5178y bearing mice showed Т/С=172%. The received results show that RrA belongs to I type cellular L-asparaginases with low L-gln activity and the high antiproliferative effect.
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Pokrovskaya M.V., Pokrovskiy V.S., Aleksandrova S.S., Anisimova N.Yu., Andrianov R.M., Treschalina E.M., Ponomarev G.V., Sokolov N.N. (2013) Biomeditsinskaya khimiya, 59(2), 192-208.
This paper is also available as the English translation:10.1134/S1990750812020096
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