The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis

   
Vasilyeva I.N.1 , Zinkin V.N.2

1. St.-Petersburg Scientific Research Institute of Phthisiopulmonology
2. State Scientific Research Tested Institute of Military Medicine, DM RF
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20135903358      PubMed Id: 23987073
Year: 2013  Volume: 59  Issue: 3  Pages: 358-373
The low-molecular-weight DNA appears in blood plasma of irradiated rats, and its content correlates directly with the irradiation dose. Cloning has shown, that enrichment of low-molecular-weight DNA with G+C content and features of its nucleotide sequences point to its ability to form rather stable nucleosomes. DNA obtained after irradiation of rats with principally different doses 8 and 100 Gy differed not only quantitatively, but also by content of the dinucleotides CpG and CpT; this suggests their origin from different sites of genome. For the first time it has been shown that exposure to low-frequency noise results in an increase of the contents of blood plasma low-molecular-weight DNA. In stroke patients blood concentrations of this DNA increased 3 days after the beginning of the acute period, and dynamics of its excretion differs in ischemic and hemorrhagic forms; in the case of ischemia low-molecular-weight DNA appears in cerebrospinal fluid. The chronic obstructive pulmonary disease in the state of remission is characterized by the decline of the level of low-molecular-weight DNA in the blood plasma unlike in the case of the chronic nonobstructive bronchitis. The clear dependence between formation and special features of the low-molecular-weight DNA fraction in blood plasma makes it possible to consider the low-molecular fraction as an universal index of apoptosis, which allows to distinguish basically different conditions of the body.
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Keywords: low-molecular-weight DNA, ionizing radiation, nucleosomes, low-frequency noise, ischemic cerebrovascular accident, chronic obstructive pulmonary disease, apoptosis
Citation:

Vasilyeva, I. N., Zinkin, V. N. (2013). The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis. Biomeditsinskaya khimiya, 59(3), 358-373.
This paper is also available as the English translation: 10.1134/S1990750812030146
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