The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis

Vasilyeva I.N.1 , Zinkin V.N.2

1. St.-Petersburg Scientific Research Institute of Phthisiopulmonology
2. State Scientific Research Tested Institute of Military Medicine, DM RF
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20135903358      PubMed Id: 23987073
Year: 2013  Volume: 59  Issue: 3  Pages: 358-373
The low-molecular-weight DNA appears in blood plasma of irradiated rats, and its content correlates directly with the irradiation dose. Cloning has shown, that enrichment of low-molecular-weight DNA with G+C content and features of its nucleotide sequences point to its ability to form rather stable nucleosomes. DNA obtained after irradiation of rats with principally different doses 8 and 100 Gy differed not only quantitatively, but also by content of the dinucleotides CpG and CpT; this suggests their origin from different sites of genome. For the first time it has been shown that exposure to low-frequency noise results in an increase of the contents of blood plasma low-molecular-weight DNA. In stroke patients blood concentrations of this DNA increased 3 days after the beginning of the acute period, and dynamics of its excretion differs in ischemic and hemorrhagic forms; in the case of ischemia low-molecular-weight DNA appears in cerebrospinal fluid. The chronic obstructive pulmonary disease in the state of remission is characterized by the decline of the level of low-molecular-weight DNA in the blood plasma unlike in the case of the chronic nonobstructive bronchitis. The clear dependence between formation and special features of the low-molecular-weight DNA fraction in blood plasma makes it possible to consider the low-molecular fraction as an universal index of apoptosis, which allows to distinguish basically different conditions of the body.
Download PDF:  
Keywords: low-molecular-weight DNA, ionizing radiation, nucleosomes, low-frequency noise, ischemic cerebrovascular accident, chronic obstructive pulmonary disease, apoptosis

Vasilyeva, I. N., Zinkin, V. N. (2013). The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis. Biomeditsinskaya Khimiya, 59(3), 358-373.
This paper is also available as the English translation: 10.1134/S1990750812030146
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)