Atomic force microscopy with two types of probes – standard (radius of curvature R~10 nm) and supersharp (R~2 nm) – was used to determine CYP102A1oligomeric state. CYP102A1 images were obtained in a liquid, air and vacuum environment using the standard probes, also a ratio of monomers to oligomers (a) of CYP102A1 were determined as a=0.48:0.52. At the same time use of standard probes did not allow to resolve the structure of these oligomers. Supersharp probes allowed to obtain the data about the monomers to oligomers ratio, and also about the dimers/trimers/tetramers ratio in air and vacuum. So, a ratio a of CYP102A1 in liquid can be determined by the standard probes, and an oligomeric state of protein can be specified by the supersharp probes.
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Keywords: atomic force microscopy, cytochrome P450 CYP102A1, oligomeric state
Citation:
Ivanov Yu.D., Bukharina N.S., Frantsuzov P.A., Pleshakova T.O., Krohin N.V., Kanashenko S.L., Archakov A.I. (2013) Oligomeric state investigation of flavocytochrome CYP102A1 using afm with standard and supersharp probes. Biomeditsinskaya Khimiya, 59(4), 378-387.
Ivanov Yu.D. et al. Oligomeric state investigation of flavocytochrome CYP102A1 using afm with standard and supersharp probes // Biomeditsinskaya Khimiya. - 2013. - V. 59. -N 4. - P. 378-387.
Ivanov Yu.D. et al., "Oligomeric state investigation of flavocytochrome CYP102A1 using afm with standard and supersharp probes." Biomeditsinskaya Khimiya 59.4 (2013): 378-387.
Ivanov, Yu. D., Bukharina, N. S., Frantsuzov, P. A., Pleshakova, T. O., Krohin, N. V., Kanashenko, S. L., Archakov, A. I. (2013). Oligomeric state investigation of flavocytochrome CYP102A1 using afm with standard and supersharp probes. Biomeditsinskaya Khimiya, 59(4), 378-387.
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