Activation of ganglioside GM3 biosynthesis in human blood mononuclear cells in atherosclerosis

Gracheva E.V.1, Samovilova N.N.1, Golovanova N.K.1, Piksina G.F.1, Shishkina V.S.1, Prokazova N.V.1

1. Research Institute of Experimental Cardiology, Cardiology Research Center of the Ministry of Public Health and Social Development
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20135904459      PubMed Id: 24502144
Year: 2013  Volume: 59  Issue: 4  Pages: 459-468
Using blood monocytes and lymphocytes from atherosclerotic patients and healthy subjects we have investigated activity of GM3 synthase, cellular levels of ganglioside GM3 and its role in monocyte adhesion to cultured human umbilical vein endothelial cells (HUVEC). The results showed that activity of GM3 synthase and cellular levels of ganglioside GM3 in blood mononuclear cells from atherosclerotic patients were several-fold higher than those from healthy subjects. In monocytes the activity of GM3 synthase was one an order of magnitude higher than in lymphocytes from both groups studied; this suggests the major contribution of monocytes to enhanced biosynthesis and levels of GM3 in mononuclear cells in atherosclerosis. Enrichment of monocytes from healthy subjects with ganglioside GM3 by incubation in medium containing this ganglioside increased adherence of these monocytes to HUVEC up to the values typical for monocytes from atherosclerotic patients. In addition, an increase in CD11b integrin expression was observed that was comparable to that seen in lipopolysaccharide-activated monocytes. It is suggested that in atherosclerosis the enhanced cellular levels of GM3 in monocytes and lymphocytes may be an important element of cell activation that facilitates their adhesion to endothelial cells and penetration into intima.
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Keywords: ganglioside GM3, GM3 synthase, blood mononuclear cells, monocytes and lymphocytes, atherosclerosis

Gracheva, E. V., Samovilova, N. N., Golovanova, N. K., Piksina, G. F., Shishkina, V. S., Prokazova, N. V. (2013). Activation of ganglioside GM3 biosynthesis in human blood mononuclear cells in atherosclerosis. Biomeditsinskaya Khimiya, 59(4), 459-468.
This paper is also available as the English translation: 10.1134/S1990750812030055
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