Antitumor activity of L-asparaginase from Erwinia Carotovora from against different leukemic and solid tumours cell lines

Abakumova O.Yu.1 , Podobed O.V.1, Karalkin P.A.1, Kondakova L.I.2, Sokolov N.N.1

1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
2. Research Institute of Human Morphology, Russian Academy of Medical Sciences
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20135905498      PubMed Id: 24479339
Year: 2013  Volume: 59  Issue: 5  Pages: 498-513
We have studied dose- and time-dependent antitumor and cytotoxic effects of Erwinia carotovora L-asparaginase (ECAR LANS) and Escherichia coli L-asparaginase (MEDAC) on human leukemic cells and human and animal solid tumor cells. We determined the sensitivity of tumor cells to L-asparaginases, as well the effect L-asparaginases on cell growth rate, protein and DNA synthesis per se and with addition of different cytostatics. The data obtained demonstrated that ECAR LANS L-asparaginase suppressed growth of all tested solid tumor cells. Evaluation of leukemic cell number after treatment with L-asparaginases for 24, 48 and 72 h demonstrated that asparagine deficiency did not kill cells but stopped normal cell division and had no effect on protein and DNA synthesis. Cytofluorometric study of solid and leukemic cells demonstrated that the treatment with L-asparaginase for 72 h did not change cell cycle phase distribution and did not increase the number of apoptotic cells. The HL-60 cell line was only exemption. At the same time, cells treatment with L-asparaginase and doxorubicin combination leaded to increase of apoptotypical cell number to 60% for MCF7 cells, to 40% for Jurkat cells and to 99% for HL-60 cells. We have excluded apoptosis as main reason for tumor cell death after asparaginase treatment because multi resistant Jurkat/A4 cells have been asparaginase sensitive. We have not found ECAR LANS L-asparaginase effect on normal human fibroblasts growth ability and we had come to conclusion that enzyme cytotoxcisity related only with asparagine deficiency.
Download PDF:  
Keywords: L-asparaginase, tumor cells, cytotoxicity, synthesis of protein and DNA, apoptosis, Erwinia carotovora

Abakumova, O. Yu., Podobed, O. V., Karalkin, P. A., Kondakova, L. I., Sokolov, N. N. (2013). Antitumor activity of L-asparaginase from Erwinia Carotovora from against different leukemic and solid tumours cell lines. Biomeditsinskaya Khimiya, 59(5), 498-513.
This paper is also available as the English translation: 10.1134/S1990750812040026
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)