Modified hplc method of determination of the valproic acid in biological fluids

   
Dutov A.A.1 , Nikitin D.A.2, Letunov V.I.2, Tereshkov P.P.1, Konovalova O.N.2

1. Chita State Medical Academy
2. Trans-Baikal State University
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20135905514      UDK: 542.98:615.322      PubMed Id: 24479340
Year: 2013  Volume: 59  Issue: 5  Pages: 514-518
Proposed modified HPLC method for determination of valproic acid in biological fluids. Created solid-phase extraction of valproic and heptanoic acids (internal standard, IS) on the cartridges packed hyper cross-linked polystyrene which maintain some tens extractions without losses of efficiency. Carboxylic acids are derivative with 1-(bromoacetyl)pyrene in acetone at presence of triethylamine. Chromatographic separation of derivatives is performed on Chromolith Performance RP-18e columns, which packed unique monolithic sorbent. UV detection at 360 nm. Mobile phase acetonitrile – water (90:10, v/v) plus 1% isopropanol, speed flow 2000 microL/min, pressure 21 bar. Complete chromatographic cycle less than 3 minutes. Yield of IS and valproic acid (extraction plus derivatization) was 101-106%. Sensitivity (limit detection) was near 1 ng for valproic and near 0.6 ng for heptanoic acid during signal/noise ratio = 3.
Download PDF:
Reference:

Dutov A.A., Nikitin D.A., Letunov V.I., Tereshkov P.P., Konovalova O.N. (2013) Biomeditsinskaya khimiya, 59(5), 514-518.
References  
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)
        

© Institute of Biomedical Chemistry, Moscow, Russia