Optimization of L-phenylalanine-ammonia-lyase liophilization

   
Babich O.O.1 , Prosekov A.Y.1

1. Kemerovo Technology Institute of Food Industry
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20135906682      PubMed Id: 24511680
Year: 2013  Volume: 59  Issue: 6  Pages: 682-692
The influence of freezing-thawing on the activity of recombinant L-phenylalanine-ammonia-lyase has been investigated. Conditions of liophilization of recombinant L-phenylalanine-ammonia-lyase at which enzyme loses about 10% of activity in a single freeze-thaw cycle are chosen. Dependence of cryoscopic temperature of enzyme from concentration of protein in a preparation is studied. The optimum temperature of freezing of enzymatic preparation L-phenylalanine-ammonia-lyase for sublimatic drying is chosen.
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Keywords: L-phenylalanine-ammonia-lyase, drying, liophilization
Citation:

Babich, O. O., Prosekov, A. Y. (2013). Optimization of L-phenylalanine-ammonia-lyase liophilization. Biomeditsinskaya Khimiya, 59(6), 682-692.
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