Optimization of L-phenylalanine-ammonia-lyase liophilization

   
Babich O.O.1 , Prosekov A.Y.1

1. Kemerovo Technology Institute of Food Industry
Section: Experimental/Clinical Study
DOI: 10.18097/pbmc20135906682      UDK: 577.112.387.2      PubMed Id: 24511680
Year: 2013  Volume: 59  Issue: 6  Pages: 682-692
The influence of freezing-thawing on the activity of recombinant L-phenylalanine-ammonia-lyase has been investigated. Conditions of liophilization of recombinant L-phenylalanine-ammonia-lyase at which enzyme loses about 10% of activity in a single freeze-thaw cycle are chosen. Dependence of cryoscopic temperature of enzyme from concentration of protein in a preparation is studied. The optimum temperature of freezing of enzymatic preparation L-phenylalanine-ammonia-lyase for sublimatic drying is chosen.
Download PDF:
Reference:

Babich O.O., Prosekov A.Y. (2013) Biomeditsinskaya khimiya, 59(6), 682-692.
References  
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)
        

© Institute of Biomedical Chemistry, Moscow, Russia