Mean platelet volume: interactions with platelet aggregation activity and glycoprotein IIb-IIIa and Ib expression levels

Khaspekova S.G.1, Zyuryaev I.T.1, Yakushkin V.V.1, Naimushin Ya.A.1, Sirotkina O.V.2, Zaytseva N.O.3, Ruda M.Ya.1, Mazurov A.V.1

1. Russian Cardiology Research and Production Complex
2. Petersburg Nuclear Physics Institute; North-Western State Medical University
3. Petersburg Nuclear Physics Institute
Section: Clinical Study
DOI: 10.18097/PBMC20146001094      PubMed Id: 24749250
Year: 2014  Volume: 60  Issue: 1  Pages: 94-108
Increased mean platelet volume (MPV) is an independent risk factor of thrombotic events in patients with cardiovascular diseases. Interactions of MPV with platelet aggregation activity and contents of glycoprotein (GP) IIb-IIIa (aIIb/b3 integrin, fibrinogen receptor) and GP Ib (von Willebrand factor receptor) were investigated in this study. Investigation was performed in a group of healthy volunteers (n = 38) and in a group of patients with acute coronary syndrome (ACS). In patients blood was collected at days 1, 3-5 and 8-12 after ACS development. As an antiaggregant therapy all patients received acetylsalicylic acid (ASA, inhibitor of thromboxane A2 synthesis) and most of them – clopidogrel (ADP receptor antagonist) with the exception of part of the patients (n=44) at day 1 who had not taken clopidogrel before first blood collection. In volunteers platelet aggregation was stimulated by 1.25, 2.5, 5 and 20 M ADP, and in patients – by 5 and 20 M ADP. GP IIb-IIIa and GP Ib content on platelet surface was measured using 125I-labelled monoclonal antibodies. GP IIb-IIIa and GP Ib genetic polymorphisms were determined in ACS patients. In healthy donors significant correlations between MPV and aggregation levels were revealed at 1.25 and 2.5 M ADP (coefficients of correlation (r) - 0.396 and 0.373, p<0.05) and at 5 and 20 those interactions did not reach significant level (r - 0.279 and 0.205, p>0.05). Correlations between MPV and aggregation levels were observed at day 1 of ACS in a subgroup of patients who received ASA but had not started clopidogrel treatment (r - 0.526, p<0.01 and 0.368, p<0.05 for 5 and 20 M ADP respectively). Interactions between these parameters were not registered upon combined treatment with ASA and clopidogrel. Strong direct correlations between MPV and GP IIb-IIIa and GP Ib contents were detected in healthy donors and ACS patients (at all time points) – r from 0.439 to 0.647 (p£0.001 for all correlations). Genetic polymorphisms of GP IIb-IIIa (GP IIIa Leu33Pro) and GP Ib ((-5)T/C ( Kozak ) and Thr145Met) identified in ACS patients did not affect expression levels of corresponding glycoproteins. The data obtained indicated that increased MPV values correlate with increased platelet aggregation activity and enhanced GP IIb-IIIa and GP Ib expression.
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Keywords: mean platelet volume, platelet aggregation, glycoprotein IIb-IIIa, glycoprotein Ib, acute coronary syndrome

Khaspekova, S. G., Zyuryaev, I. T., Yakushkin, V. V., Naimushin, Ya. A., Sirotkina, O. V., Zaytseva, N. O., Ruda, M. Ya., Mazurov, A. V. (2014). Mean platelet volume: interactions with platelet aggregation activity and glycoprotein IIb-IIIa and Ib expression levels. Biomeditsinskaya Khimiya, 60(1), 94-108.
This paper is also available as the English translation: 10.1134/S199075081402005X
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