From the highly purified but lowly active recombinant protein Destabilas-Lysozyme (Dest-Lys) by use cation-exchange column TSK CM 3-SW chromatography, it was separated non-active fraction IV, contained 90% of protein. Fractions I, II and III, represented proteins with lysozyme and isopeptidase activities. Their lysozyme activity correlates with the activity of natural Des-Lys. The ratio of the activities in fractions I – III is such, that maximal lysozyme activity is concentrated in fraction III, isopeptidase – in fraction I. It is discussed the possibility of Dest-Lys different functions regulation is depended on the formation of protein complex forms
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Keywords: destabilase-lysozyme of Hirudo medicinalis, lysozyme and isopeptidase functions, forms of recombinant enzyme
Citation:
Fadeeva Yu.I., Antipova N.V., Baskova I.P., Zavalova L.L. (2014) Highly active fractions of the medicinal leech recombinant destabilase-lysozyme. Biomeditsinskaya Khimiya, 60(3), 332-337.
Fadeeva Yu.I. et al. Highly active fractions of the medicinal leech recombinant destabilase-lysozyme // Biomeditsinskaya Khimiya. - 2014. - V. 60. -N 3. - P. 332-337.
Fadeeva Yu.I. et al., "Highly active fractions of the medicinal leech recombinant destabilase-lysozyme." Biomeditsinskaya Khimiya 60.3 (2014): 332-337.
Fadeeva, Yu. I., Antipova, N. V., Baskova, I. P., Zavalova, L. L. (2014). Highly active fractions of the medicinal leech recombinant destabilase-lysozyme. Biomeditsinskaya Khimiya, 60(3), 332-337.
Zavalova L., Lukyanov S., Baskova I., Snezhkov E., Akopov S., Berezhnoy S., Bogdanova, E., Barsova E., Sverdlov E. (1996) Mol. Gen. Genet., 253, 20–25. CrossRef Scholar google search
