Search of potential gastric cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry

Shevchenko V.E.1 , Arnotskaya N.E.1, Ogorodnikova E.V.1, Davidov M.M.1, Ibraev M.A.1, Turkin I.N.1, Davidov M.I.1

1. N.N. Blokhin Russian Cancer Research Center of Russian Academy of Medical Sciences
Section: Clinical Study
DOI: 10.18097/pbmc20146004503      UDK: 616-006.6      PubMed Id: 25249535
Year: 2014  Volume: 60  Issue: 4  Pages: 503-514
Gastric cancer, one of the most widespread malignant tumors, still lacks reliable serum/plasma biomarkers of its early detection. In this study we have developed, unified, and tested a new methodology for search of gastric cancer biomarkers based on profiling of low molecular weight proteome (LMWP) (1-17 kDa). This approach included three main components: sample pre-fractionation, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), data analysis by a bioinformatics software package. Applicability and perspectives of the developed approach for detection of potential gastric cancer markers during LMWP analysis have been demonstrated using 69 plasma samples from patients with gastric cancer (stages I-IV) and 238 control samples. The study revealed peptides/polypeptides, which may be potentially used for detection of this pathology.
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Shevchenko V.E., Arnotskaya N.E., Ogorodnikova E.V., Davidov M.M., Ibraev M.A., Turkin I.N., Davidov M.I. (2014) Biomeditsinskaya khimiya, 60(4), 503-514.
This paper is also available as the English translation:10.1134/S1990750813040094
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