Conjugates of streptavidin with gold nanoparticles for the visualization of dna single interactions on the silicon surface

   
Presnova G.V.1, Rubtsova M.Yu.1 , Presnov D.E.2, Grigorenko V.G.1, Yaminsky I.V.3, Egorov A.M.1

1. M.V. Lomonosov Moscow State University; RPE ”Immunotek”
2. Skobeltsyn Institute of Nuclear Physics, M.V. Lomonosov Moscow State University
3. Advanced Technologies Center, Moscow
Section: Experimental Study
DOI: 10.18097/pbmc20146005538      UDK: 577.29; 539.25; 546.05      PubMed Id: 25386881
Year: 2014  Volume: 60  Issue: 5  Pages: 538-542
The potential of the method of scanning electron microscopy (SEM) to visualize the results of individual acts of DNA and oligonucleotides hybridization using gold nanoparticles as label was investigated. Molecule of biotin was introduced into DNA or oligonucleotide, and then it was detected in DNA duplex using a conjugate of streptavidin with gold nanoparticles. Effective imaging of DNA duplexes was possible using a conjugate prepared by covalent binding.. The detection limit of the model oligonucleotide of 19 bases was 20 pg.
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Presnova G.V., Rubtsova M.Yu., Presnov D.E., Grigorenko V.G., Yaminsky I.V., Egorov A.M. (2014) Biomeditsinskaya khimiya, 60(5), 538-542.
This paper is also available as the English translation:10.1134/S1990750814020103
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