1. M.V. Lomonosov Moscow State University; RPE ”Immunotek” 2. Skobeltsyn Institute of Nuclear Physics, M.V. Lomonosov Moscow State University 3. Advanced Technologies Center, Moscow
The potential of the method of scanning electron microscopy (SEM) to visualize the results of individual acts of DNA and oligonucleotides hybridization using gold nanoparticles as label was investigated. Molecule of biotin was introduced into DNA or oligonucleotide, and then it was detected in DNA duplex using a conjugate of streptavidin with gold nanoparticles. Effective imaging of DNA duplexes was possible using a conjugate prepared by covalent binding.. The detection limit of the model oligonucleotide of 19 bases was 20 pg.
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Keywords: hybridization analysis of DNA, single biospecific interactions, gold nanoparticles, scanning electron microscopy
Citation:
Presnova G.V., Rubtsova M.Yu., Presnov D.E., Grigorenko V.G., Yaminsky I.V., Egorov A.M. (2014) Conjugates of streptavidin with gold nanoparticles for the visualization of dna single interactions on the silicon surface. Biomeditsinskaya Khimiya, 60(5), 538-542.
Presnova G.V. et al. Conjugates of streptavidin with gold nanoparticles for the visualization of dna single interactions on the silicon surface // Biomeditsinskaya Khimiya. - 2014. - V. 60. -N 5. - P. 538-542.
Presnova G.V. et al., "Conjugates of streptavidin with gold nanoparticles for the visualization of dna single interactions on the silicon surface." Biomeditsinskaya Khimiya 60.5 (2014): 538-542.
Presnova, G. V., Rubtsova, M. Yu., Presnov, D. E., Grigorenko, V. G., Yaminsky, I. V., Egorov, A. M. (2014). Conjugates of streptavidin with gold nanoparticles for the visualization of dna single interactions on the silicon surface. Biomeditsinskaya Khimiya, 60(5), 538-542.
