Biocompatibility of electrospun poly(3-hydroxybutyrate) and its composites scaffoldsfor tissue engineering

Zharkova I.I.1 , Staroverova O.V.2, Voinova V.V.1, Andreeva N.V.1, Shushckevich A.M.3, Sklyanchuk E.D.3, Kuzmicheva G.M.2, Bespalova A.E.1, Akulina E.A.1, Shaitan K.V.1, Olkhov A.A.2

1. Lomonosov Moscow State University
2. Lomonosov Moscow State University of Fine Chemical Technologies
3. Moscow State University of Medicine and Dentistry
Section: Experimental Study
DOI: 10.18097/pbmc20146005553      UDK: 57.086.33; 615.40.54      PubMed Id: 25386884
Year: 2014  Volume: 60  Issue: 5  Pages: 553-560
Development of biodegradable polymers-based scaffolds for tissue engineering is a promising trend in bioengineering. The electrospun scaffolds from poly(3-hydroxybutyrate) (PHB) were produced using different additives that changed the physical and chemical characteristics of the products. As a result, the construct consisting of interwoven threads of different diameter (0.8-3.4 mm) were obtained, the smallest diameter was observed in the threads from the PHB using tetrabutilammonium iodide (TBAI) and titanium oxide II (TiO2) as additives. Mesenchymal stem cells (MSC) were cultivated on the scaffolds for the biocompatibility evaluation of obtained materials. Cells viability was determined by the XTT assay test. It was shown that the scaffold from the interwoven threads of lowest diameter is most favorable for MSC growth in comparison with the polymer film and scaffolds from the threads of larger diameter. Thus, it was shown that the biocompatibility of electrospun PHB scaffolds depended on their microstructure. The obtained data can be used for development of scaffolds for tissue engineering.
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Zharkova I.I., Staroverova O.V., Voinova V.V., Andreeva N.V., Shushckevich A.M., Sklyanchuk E.D., Kuzmicheva G.M., Bespalova A.E., Akulina E.A., Shaitan K.V., Olkhov A.A. (2014) Biomeditsinskaya khimiya, 60(5), 553-560.
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