We have developed a simple HPLC method for analysis of the dehydroepiandrosterone sulfate (DHEA-sulfate) in serum with use a new procedure of solid-phase extraction (SPE) on hyper cross-linked polystyrene (Purosep-200) and fast chromatographic separation on the monolithic column under isocratic elution and UV detection at 200 nm. Complete SPE procedure lasts for about 7 min, chromatographic separation takes less than 6 min. Simplicity and high reproducibility of this method makes it attractive in routine clinical practice.
Dutov A.A., Nikitin D.A., Lukyanova Yu.L., Sverkunova A.V., Martinova A.V., Ermolina A.V. (2014) HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200). Biomeditsinskaya Khimiya, 60(6), 651-654.
Dutov A.A. et al. HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200) // Biomeditsinskaya Khimiya. - 2014. - V. 60. -N 6. - P. 651-654.
Dutov A.A. et al., "HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200)." Biomeditsinskaya Khimiya 60.6 (2014): 651-654.
Dutov, A. A., Nikitin, D. A., Lukyanova, Yu. L., Sverkunova, A. V., Martinova, A. V., Ermolina, A. V. (2014). HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200). Biomeditsinskaya Khimiya, 60(6), 651-654.
