HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200)

   
Dutov A.A.1 , Nikitin D.A.2, Lukyanova Yu.L.1, Sverkunova A.V.2, Martinova A.V.2, Ermolina A.V.1

1. State Medical Academy
2. Zabaikalsky State University, Chita, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20146006651      UDK: 542,98: 615,322      PubMed Id: 25552504
Year: 2014  Volume: 60  Issue: 6  Pages: 651-654
We have developed a simple HPLC method for analysis of the dehydroepiandrosterone sulfate (DHEA-sulfate) in serum with use a new procedure of solid-phase extraction (SPE) on hyper cross-linked polystyrene (Purosep-200) and fast chromatographic separation on the monolithic column under isocratic elution and UV detection at 200 nm. Complete SPE procedure lasts for about 7 min, chromatographic separation takes less than 6 min. Simplicity and high reproducibility of this method makes it attractive in routine clinical practice.
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Dutov A.A., Nikitin D.A., Lukyanova Yu.L., Sverkunova A.V., Martinova A.V., Ermolina A.V. (2014) Biomeditsinskaya khimiya, 60(6), 651-654.
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