In vitro protein phosphorylation as a template for SRM method development

Zavialova M.G.1, Zgoda V.G.1 , Kharybin O.N.1, Nikolayev E.N.2

1. Orekhovich Institute of Biomedical Chemistry (IBMC)
2. Orekhovich Institute of Biomedical Chemistry (IBMC); Institute for Energy Problems of Chemical Physics RAS, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20146006668      PubMed Id: 25552507
Year: 2014  Volume: 60  Issue: 6  Pages: 668-676
Phosphorylation is one of the most common posttranslational modification (PTM) of proteins. Main challenge of phosphoprotein detection is their low abundance comparing to abundance of unmodified proteins. The method of selected reactions monitoring (SRM) allows to perform very sensitive and selective analysis of desired PTMs. Using myelin basic protein (MBP) as a model we have developed a method for phosphoprotein detection by SRM. The method is based on obtaining of phosphoproteins in a reconstituted kinase system and following usage these phosphorylated protein as a template for the development of the SRM method. The developed method was successfully applied for detection of phosphopeptides of myelin basic protein in the samples of human brain glioma.
Download PDF:  
Keywords: proteomics, selected reaction monitoring (SRM), post-translational modifications (PTM), protein phosphorylation

Zavialova, M. G., Zgoda, V. G., Kharybin, O. N., Nikolayev, E. N. (2014). In vitro protein phosphorylation as a template for SRM method development. Biomeditsinskaya Khimiya, 60(6), 668-676.
This paper is also available as the English translation: 10.1134/S1990750815040095
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)