HPLC estimation of coenzyme Q10 redox status in plasma after intravenous coenzyme Q10 administration

Kalenikova E.I.1 , Kharitonova E.V.2, Gorodetskaya E.A.1, Tokareva O.G.2, Medvedev O.S.2

1. Lomonosov Moscow State University, Moscow, Russia; Russian Cardiology Research Center and Production Complex, Moscow, Russia
2. Lomonosov Moscow State University, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20156101125      UDK: 615.032; 615.033      PubMed Id: 25762606
Year: 2015  Volume: 61  Issue: 1  Pages: 125-131
The pharmacokinetics of the total pool of coenzyme Q10 (CoQ10), its oxidized (ubiquinone) and reduced (ubiquinol, CoQ10H2) forms have been investigated in rats plasma during 48 h after a single intravenous injection of a solution of solubilized CoQ10 (10 mg/kg) to rats. Plasma levels of CoQ10 were determined by HPLC with spectrophotometric and coulometric detection. In plasma samples taken during the first minutes after the CoQ10 intravenous injection, the total pool of coenzyme Q10 and proportion of CoQ10H2 remained unchanged during two weeks of storage at -20°C. The kinetic curve of the total pool of coenzyme Q10 corresponds to a one-part model (R2 = 0.9932), while the corresponding curve of its oxidized form fits to the two-part model. During the first minutes after the injection a significant portion of plasma ubiquinone undergoes reduction, and after 7 h the concentration of ubiquinol predominates. The decrease in the total plasma coenzyme Q10 content was accompanied by the gradual increase in plasma ubiquinol, which represented about 90% of total plasma CoQ10 by the end of the first day. The results of this study demonstrate the ability of the organism to transform high concentrations of the oxidized form of CoQ10 into the effective antioxidant (reduced) form and justify prospects of the development of parenteral dosage forms of CoQ10 for the use in the treatment of acute pathological conditions.
Download PDF:

Kalenikova E.I., Kharitonova E.V., Gorodetskaya E.A., Tokareva O.G., Medvedev O.S. (2015) Biomeditsinskaya khimiya, 61(1), 125-131.
This paper is also available as the English translation:10.1134/S199075081403007X
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)

© Institute of Biomedical Chemistry, Moscow, Russia