The effect of daily intragastric administration of an aqueous dispersion of silicon nanoparticles (NPs) (the dose range from 1.0 mg/kg to 100 mg/kg body weight for 28 days) to rats on the proteomic profile of liver microsomes has been investigated by 2D-electrophoresis followed by subsequent mass spectrometry identification. The liver microsomal fraction was isolated by differential centrifugation and its protein composition was analyzed by 2D-polyacrylamide gel electrophoresis. Identification of protein spots was carried out using MALDI-TOF mass spectrometric analysis. The mass spectrometry analysis revealed the protein GRP78 (78 kD glucose-regulated protein precursor), belonging to the family of heat shock proteins. This protein present in animals of the control group was not detected in NP-treated rats of group 2 (1 mg/kg body weight/day) and group 3 (10 mg/kg body weight/day). This protein predominantly localized in the liver cell endoplasmic reticulum and plasma membrane has the chaperone biological activity. Possible mechanisms of the effects of engineered nanoparticles on biosynthetic processes in the body are discussed.
Tananova O.N., Arianova E.A., Gmoshinskii I.V., Toropygin I.Yu., Khryapova E.V., Trusov N.V., Khotimchenko S.A., Tutel'yan V.A. (2015) Changes in proteome profiles of rat liver microsomes induced by silicon dioxide nanoparticles. Biomeditsinskaya Khimiya, 61(1), 92-98.
Tananova O.N. et al. Changes in proteome profiles of rat liver microsomes induced by silicon dioxide nanoparticles // Biomeditsinskaya Khimiya. - 2015. - V. 61. -N 1. - P. 92-98.
Tananova O.N. et al., "Changes in proteome profiles of rat liver microsomes induced by silicon dioxide nanoparticles." Biomeditsinskaya Khimiya 61.1 (2015): 92-98.
Tananova, O. N., Arianova, E. A., Gmoshinskii, I. V., Toropygin, I. Yu., Khryapova, E. V., Trusov, N. V., Khotimchenko, S. A., Tutel'yan, V. A. (2015). Changes in proteome profiles of rat liver microsomes induced by silicon dioxide nanoparticles. Biomeditsinskaya Khimiya, 61(1), 92-98.
Yang X., Liu J., He H., Zhou L., Gong C., Wang X., Yang L., Yuan J., Huang H., He L., Zhang B., Zhuang Z. (2010) Part Fibre Toxicol., 7, 1-12. CrossRef Scholar google search
Kanaeva I.P., Petushkova N.A., Lokhov P.G., Zgoda V.G., Karuzina I.I., Lisitsa A.V., Archakov A.I., Study of the mouse liver microsomes by the methods of proteome analysis, Biomeditsinskaya khimiya, 2004, vol: 50(4), 367-375. Scholar google search
“Edinye sanitarno-ehpidemiologicheskie i gigienicheskie trebovaniya k tovaram, podlezhashhim sanitarno-ehpidemiologicheskomu nadzoru (kontrolyu)”, utverzhdyon Resheniem Komissii Tamozhennogo soyuza №299 ot 28 maya 2010. Scholar google search
Lake B.G. (1987) Biochem. Toxicol.: A Practical Approach. – Oxford, pp. 183-215. Scholar google search
Shim W., Paik M.J., Nguyen D.T., Lee J.K., Lee Y., Kim J.H., Shin E.H., Kang J.S., Jung H.S., Choi S., Park S., Shim J.S., Lee G. (2012) ACS Nano, 9, 7665-7680. CrossRef Scholar google search
Ji C., Kaplowitz N., Lau M.Y., Kao E., Petrovic L.M., Lee A.S. (2011) Hepatology, 54(1), 229-239. CrossRef Scholar google search
Shi J., Karlsson H.L., Johansson K., Gogvadze V., Xiao L., Li J., Burks T., Garcia-Bennett A., Uheida A., Muhammed M., Mathur S., Morgenstern R., Kagan V.E., Fadeel B. (2012) ACS Nano, 6(3), 1925-1938. CrossRef Scholar google search
