The proteome profile of Danio rerio embryos grown in the medium containing doxorubicin, included in the phospholipid transport nanosystem (doxolip) has been investigated using combination of 1D-electrophoresis with subsequent MALDI-TOF-PMF mass spectrometry. Cultivation of growing of D. rerio embryos in the medium with doxolip caused a substantial increase in expression of the cytoskeletal proteins, a decrease in the number of nuclear proteins involved in DNA and RNA synthesis and disappearance of vitellogenin 2 in comparison with control (the cultivation medium containing the phospholipid transport nanosystem). Analysis of the proteomic profiles of doxolip-treated embryos suggests lower toxicity of doxorubicin incorporated in the phospholipid nanosystem.
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Keywords: doxorubicin, doxolip, Danio rerio, one-dimensional gel electrophoresis, mass spectrometry, phospholipid nanoparticles
Citation:
Samenkova N.F., Kisrieva Y.S., Petushkova N.A., Kuznetsova G.P., Larina O.V., Trifonova O.P., Karuzina I.I., Ipatova O.M., Lisitsa A.V. (2015) Analysis of proteomic profile changes of zebrafish embryos during exposure to doxorubicin, built-in the phospholipid transport nanosystem. Biomeditsinskaya Khimiya, 61(3), 350-356.
Samenkova N.F. et al. Analysis of proteomic profile changes of zebrafish embryos during exposure to doxorubicin, built-in the phospholipid transport nanosystem // Biomeditsinskaya Khimiya. - 2015. - V. 61. -N 3. - P. 350-356.
Samenkova N.F. et al., "Analysis of proteomic profile changes of zebrafish embryos during exposure to doxorubicin, built-in the phospholipid transport nanosystem." Biomeditsinskaya Khimiya 61.3 (2015): 350-356.
Samenkova, N. F., Kisrieva, Y. S., Petushkova, N. A., Kuznetsova, G. P., Larina, O. V., Trifonova, O. P., Karuzina, I. I., Ipatova, O. M., Lisitsa, A. V. (2015). Analysis of proteomic profile changes of zebrafish embryos during exposure to doxorubicin, built-in the phospholipid transport nanosystem. Biomeditsinskaya Khimiya, 61(3), 350-356.
Ipatova O.M., Torkhovskaya T.I., Medvedeva N.V., Prozorovskiy V.N., Ivanova N.D., Shironin A.V., Baranova V.S., Archakov A.I., Bioavailability of oral drug formulations and methods for its improvement, Biomeditsinskaya khimiya, 2010, 56(1), 101-119. CrossRef Scholar google search
