Allele polymorphism analysis in coagulation factors F2, F5 and folate metabolism gene MTHFR by using microchip-based multiplex real time PCR

   
Bogdanov K.V.1 , Nikitin M.M.2, Slyadnev M.N.2

1. Institute of Hematology, V.A. Almazov Federal Medical Research Centre, St. Petersburg, Russia; Lumex Group, St. Petersburg, Russia
2. St. Petersburg State University, St. Petersburg, Russia; Lumex Group, St. Petersburg, Russia
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DOI: 10.18097/PBMC20156103357      UDK: 577.2      PubMed Id: 26215413
Year: 2015  Volume: 61  Issue: 3  Pages: 357-362
Single nucleotide polymorphism (SNP) genotyping methods are widely used for the detection of hereditary thrombophilias caused by genetic defects in the coagulation system. The hereditary thrombophilias are frequently associated with higher incidences of point mutations in hemostasis (F2 20210G>A, F5 1691G>A) and folate metabolism (MTHFR 677C>Т, MTHFR 1298A>C) genes. Moreover, the combination of gene abnormalities in F2 or/and MTHFR with F5 Leiden mutation leads to increased risk of developing thrombosis. Thus, simultaneous detection of the multiple gene mutations in a sample has important clinical relevance. The microchip-based multiplex real time PCR for estimation of allele specific polymorphism in hemostatic and folate metabolism genes presented here has a high efficiency and may be used for laboratory diagnosis. The optimized protocol for estimation of 4 different types of genetic polymorphisms allowed PCR to be performed with minimal quantity of DNA template and PCR reagents including Taq polymerase and a short-term thermocycling.
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Bogdanov K.V., Nikitin M.M., Slyadnev M.N. (2015) Biomeditsinskaya khimiya, 61(3), 357-362.
This paper is also available as the English translation:10.1134/S1990750816020025
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