Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification

Bukharina N.S.1 , Ivanov Yu.D.1, Pleshakova T.O.1, Frantsuzov P.A.1, Andreeva E.Yu.1, Kaysheva A.L.1, Izotov A.A.1, Pavlova T.I.1, Ziborov V.S.1, Radko S.P.1, Archakov A.I.1

1. Institute of Biomedical Chemistry, Moscow, Russia
DOI: 10.18097/PBMC20156103363      PubMed Id: 26215414
Year: 2015  Volume: 61  Issue: 3  Pages: 363-372
A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.
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Keywords: gp120 HIV-1, aptamer, atomic force microscopy, fishing, mass spectrometry

Bukharina, N. S., Ivanov, Yu. D., Pleshakova, T. O., Frantsuzov, P. A., Andreeva, E. Yu., Kaysheva, A. L., Izotov, A. A., Pavlova, T. I., Ziborov, V. S., Radko, S. P., Archakov, A. I. (2015). Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification. Biomeditsinskaya Khimiya, 61(3), 363-372.
This paper is also available as the English translation: 10.1134/S1990750814020036
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