SOI-nanowire biosensor for the detection of D-NFAT 1 protein

Malsagova K.A.1, Ivanov Yu.D.1, Pleshakova T.O.1, Kozlov A.F.1, Krohin N.V.1, Kaysheva A.L.1, Shumov I.D.1 , Popov V.P.2, Naumova O.V.2, Fomin B.I.2, Nasimov D.A.2

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia
Section: Experimental/Clinical Study
DOI: 10.18097/PBMC20156104462      PubMed Id: 26350736
Year: 2015  Volume: 61  Issue: 4  Pages: 462-467
The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes. It was shown that using this biosensor it is possible to reach the sensitivity of ~10-15 M. This sensitivity was comparable with that of the NW biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate promising approaches used to form the sensor elements for high-sensitive disease diagnostics
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Keywords: aptamer, biosensor, D-NFAT 1, silicon-on-insulator, nanowires

Malsagova, K. A., Ivanov, Yu. D., Pleshakova, T. O., Kozlov, A. F., Krohin, N. V., Kaysheva, A. L., Shumov, I. D., Popov, V. P., Naumova, O. V., Fomin, B. I., Nasimov, D. A. (2015). SOI-nanowire biosensor for the detection of D-NFAT 1 protein. Biomeditsinskaya khimiya, 61(4), 462-467.
This paper is also available as the English translation: 10.1134/S199075081403010X
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