1. Research Institute for Clinical and Experimental Rheumatology RAMS, Volgograd, Russia 2. Volgograd State Medical University, Volgograd, Russia 3. Research Institute for Clinical and Experimental Rheumatology RAMS
The objective of this research was to adapt the experimental model simulating the nucleoprotein disposal disorders in systemic lupus erythematosus (SLE) for further study of its extracorporeal correction, as well as to assess validity of the model by short-term experiment. Twenty to female Wistar rats were intraperitoneally injected with the chromatin-containing extract from bovine liver followed by intravenous administration of anti-DNA antibodies derived from SLE patients. After these procedures plasma concentrations of anti-dsDNA, circulating immune complexes and DNA became sharply increased, together with distinct elevation of leukocytes. On the contrary, changes in erythrocytes, platelets, total protein concentration, creatinine, asparagine and alanine aminotransferase activities, as well as blood coagulation time were changed insignificantly. Using direct immunofluorescence of cryosections, we detected human IgG deposition in rat kidneys treated in accordance with the simulation protocol. Thus, our model reproduces essential DNA disposal disorders in SLE without any animal death or the life-threatening changes in examined markers during short-term experiment.
Keywords: systemic lupus erythematosus, biosimulation, anti-DNA antibodies, chromatin, DNase I
Trofimenko A.S. et al. Experimental modeling of nucleoprotein disposal disorders in systemic lupus erythematosus // Biomeditsinskaya Khimiya. - 2015. - V. 61. -N 5. - P. 617-621.
Trofimenko A.S. et al., "Experimental modeling of nucleoprotein disposal disorders in systemic lupus erythematosus." Biomeditsinskaya Khimiya 61.5 (2015): 617-621.
Trofimenko, A. S., Gontar, I. P., Paramonova, O. V., Simakova, E. S., Zborovskaya, I. A. (2015). Experimental modeling of nucleoprotein disposal disorders in systemic lupus erythematosus. Biomeditsinskaya Khimiya, 61(5), 617-621.