1. Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia 2. Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia; Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia 3. Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia 4. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia 5. Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia
Bone tissue damages are one of the dominant causes of temporary disability and developmental disability. Currently, there are some methods of guided bone regeneration employing different osteoplastic materials and insulation membranes used in surgery. In this study, we have developed a method of preparation of porous membranes from the biopolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), produced by a strain of Azotobacter chroococcum 7B. The biocompatibility of the porous membranes was investigated in vitro using mesenchymal stem cells (MSCs) and in vivo on laboratory animals. The cytotoxicity test showed the possibility of cell attachment on membrane and histological studies confirmed good insulating properties the material. The data obtained demonstrate the high biocompatibility and the potential application of insulating membranes based on PHBV in bone tissue engineering.
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Keywords: poly(3-hydroxybutyrate-co-3-hydroxyvalerate), isolating membrane, porous scaffold, guided bone regeneration, bone tissue
Citation:
Ivanov S.Yu., Bonartsev A.P., Gazhva Yu.V., Zharkova I.I., Mukhametshin R.F., Mahina T.K., Myshkina V.L., Bonartseva G.A., Voinova V.V., Andreeva N.V., Akulina E.A., Kharitonova E.S., Shaitan K.V., Muraev A.A. (2015) Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration. Biomeditsinskaya Khimiya, 61(6), 717-723.
Ivanov S.Yu. et al. Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration // Biomeditsinskaya Khimiya. - 2015. - V. 61. -N 6. - P. 717-723.
Ivanov S.Yu. et al., "Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration." Biomeditsinskaya Khimiya 61.6 (2015): 717-723.
Ivanov, S. Yu., Bonartsev, A. P., Gazhva, Yu. V., Zharkova, I. I., Mukhametshin, R. F., Mahina, T. K., Myshkina, V. L., Bonartseva, G. A., Voinova, V. V., Andreeva, N. V., Akulina, E. A., Kharitonova, E. S., Shaitan, K. V., Muraev, A. A. (2015). Development and preclinical studies of insulating membranes based on poly-3-hydroxybutyrate-co-3-hydroxyvalerate for guided bone regeneration. Biomeditsinskaya Khimiya, 61(6), 717-723.
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