Proteomic analysis of exhaled breath condensate for diagnosis of pathologies of the respiratory system

   
Kononikhin A.S.1 , Fedorchenko K.Yu.2, Ryabokon A.M.2, Starodubtseva N.L.1, Popov I.A.1, Zavialova M.G.3, Anaev E.C.4, Chuchalin A.G.4, Varfolomeev S.D.2, Nikolaev E.N.5

1. Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia; Emanuel Institute of Biochemical Physics RAS, Moscow, Russia
2. Emanuel Institute of Biochemical Physics RAS, Moscow, Russia; Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia
3. Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia
4. Research Institute of Pulmonology, Moscow, Russia
5. Emanuel Institute of Biochemical Physics RAS, Moscow, Russia
Section: Short Communication
DOI: 10.18097/PBMC20156106777      PubMed Id: 26716752
Year: 2015  Volume: 61  Issue: 6  Pages: 777-780
Study of the proteomic composition of exhaled breath condensate (EBC), is a promising non-invasive method for the diagnosis of the respiratory tract diseases in patients. In this study the EBC proteomic composition of the 79 donors, including patients with different pathologies of the respiratory system has been investigated. Cytoskeletal keratins type II (1, 2, 3, 4, 5, 6) and cytoskeletal keratins the type I (9, 10, 14, 15, 16) were invariant for all samples. Analyzing the frequency of occurrence of proteins in different groups of examined patients, several categories of protein have been recognized: found in all pathologies (Dermcidin, Alpha-1-microglobulin, SHROOM3), found in several pathologies (CSTA, LCN1, JUP, PIP, TXN), and specific for a single pathology (PRDX1, Annexin A1/A2). The EBC analysis by HPLC-MS/MS can be used to identify potential protein markers characteristic for pathologies such as chronic obstructive pulmonary disease (PRDX1) and pneumonia (Annexin A1/A2).
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Keywords: exhaled breath condensate, mass spectrometry, proteomics, chronic obstructive pulmonary disease (COPD), pneumonia
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Kononikhin, A. S., Fedorchenko, K. Yu., Ryabokon, A. M., Starodubtseva, N. L., Popov, I. A., Zavialova, M. G., Anaev, E. C., Chuchalin, A. G., Varfolomeev, S. D., Nikolaev, E. N. (2015). Proteomic analysis of exhaled breath condensate for diagnosis of pathologies of the respiratory system. Biomeditsinskaya khimiya, 61(6), 777-780.
This paper is also available as the English translation: 10.1134/S1990750816030069
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