Activity of liver mitochondrial NAD+-dependent dehydrogenases of the krebs cycle in rats with acetaminophen-induced hepatitis developed under conditions of alimentary protein deficiency
Voloshchuk O.N.1 , Kopylchuk G.P.1
1. Fedkovych Chernovtsi National University, Chernovtsi, Ukraine
Activity of isocitrate dehydrogenase, a-ketoglutarate dehydrogenase, malate dehydrogenase, and the NAD+/NADН ratio were studied in the liver mitochondrial fraction of rats with toxic hepatitis induced by acetaminophen under conditions of alimentary protein deprivation. Acetaminophen-induced hepatitis was characterized by a decrease of isocitrate dehydrogenase, a-ketoglutarate dehydrogenase and malate dehydrogenase activities, while the mitochondrial NAD+/NADН ratio remained at the control level. Modeling of acetaminophen-induced hepatitis in rats with alimentary protein caused a more pronounced decrease in the activity of NAD+-dependent dehydrogenases studied and a 2.2-fold increase of the mitochondrial NAD+/NADН ratio. This suggests that alimentary protein deprivation potentiated drug-induced liver damage
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Keywords: alimentary protein deprivation, acetaminophen-induced hepatitis, isocitrate dehydrogenase, a-ketoglutarate dehydrogenase, malate dehydrogenase, the NAD+/NADH ratio
Citation:
Voloshchuk O.N., Kopylchuk G.P. (2016) Activity of liver mitochondrial NAD+-dependent dehydrogenases of the krebs cycle in rats with acetaminophen-induced hepatitis developed under conditions of alimentary protein deficiency. Biomeditsinskaya Khimiya, 62(2), 169-172.
Voloshchuk O.N. et al. Activity of liver mitochondrial NAD+-dependent dehydrogenases of the krebs cycle in rats with acetaminophen-induced hepatitis developed under conditions of alimentary protein deficiency // Biomeditsinskaya Khimiya. - 2016. - V. 62. -N 2. - P. 169-172.
Voloshchuk O.N. et al., "Activity of liver mitochondrial NAD+-dependent dehydrogenases of the krebs cycle in rats with acetaminophen-induced hepatitis developed under conditions of alimentary protein deficiency." Biomeditsinskaya Khimiya 62.2 (2016): 169-172.
Voloshchuk, O. N., Kopylchuk, G. P. (2016). Activity of liver mitochondrial NAD+-dependent dehydrogenases of the krebs cycle in rats with acetaminophen-induced hepatitis developed under conditions of alimentary protein deficiency. Biomeditsinskaya Khimiya, 62(2), 169-172.
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