Ability of fractions of cobra’s (Naja oxiana Eichwald) and copperhead snake’s (Agkistrodon halys halys) venoms to hydrolyze fibrinogen/fibrin was studied. In cobra’s snake a component with molecular mass of nearly 60 kDa was found to hydrolyze a-chain of fibrinogen but failed to hydrolyze casein/azocasein and fibrin. A fibrinogen-specific metalloproteinase, the enzyme was inhibited by EDTA. Cobra’s venom reduced the mass of donor’s fresh blood clots. The copperhead snake’s venom and the fractions obtained by gel-filtration (HW-50) and ion exchange chromatography (DEAE-650) were found to hydrolyze casein/azocasein, a- and b-chains of fibrinogen/fibrin and donor’s blood clots. The results from the study of the venom and proteolytically active fractions are the evidence for a thrombolytic potential in a copperhead snake’s venom.
Yunusova E.S. et al. Fibrinogen/fibrin-specific enzymes from copperhead (Agkistrodon halys halys) and cobra (Naja oxiana eichwald) snake venoms // Biomeditsinskaya Khimiya. - 2016. - V. 62. -N 3. - P. 259-264.
Yunusova E.S. et al., "Fibrinogen/fibrin-specific enzymes from copperhead (Agkistrodon halys halys) and cobra (Naja oxiana eichwald) snake venoms." Biomeditsinskaya Khimiya 62.3 (2016): 259-264.
Yunusova, E. S., Sadykov, E. S., Sultanalieva, N. M., Shkinev, A. V. (2016). Fibrinogen/fibrin-specific enzymes from copperhead (Agkistrodon halys halys) and cobra (Naja oxiana eichwald) snake venoms. Biomeditsinskaya Khimiya, 62(3), 259-264.
Fox J.W., Bjarnason J.B. (1998) in: Enzymes from snake venom (Bailey G.S., ed.), Alaken Inc., pp. 599-633. Scholar google search
Swenson S., Toombs C.F., Pena L., Johansson J., Markland F.S. Jr. (2004) Current Drug Targets - Cardiovascular & Haematological Disorders, 4, 417-435. Scholar google search
Cintra A.C.O., De Toni L.G.B., Sartim M.A., Franco J.J., Caetano R.C., Murakami M.T., Sampaio S.V. (2012) Toxicon, 60, 70-82. CrossRef Scholar google search
Fermentnye preparaty dlya pishhevoj promyshlennosti. Metody opredeleniya proteoliticheskoj aktivnosti. Natsional'nyj standart RF GOST R 53974-2010. M.: Standartinform,. 9 s. Scholar google search
Siigur J., Samel M., Tõnismägi K., Subbi J., Siigur E., Tu A.T. (1998) Thromb. Res., 90, 39-49. CrossRef Scholar google search
