Fibrinogen/fibrin-specific enzymes from copperhead (Agkistrodon halys halys) and cobra (Naja oxiana eichwald) snake venoms

Yunusova E.S.1, Sadykov E.S.1, Sultanalieva N.M.1, Shkinev A.V.1

1. Sadykov Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, Republic of Uzbekistan
Section: Experimental Study
DOI: 10.18097/PBMC20166203259      UDK: 577.1:615.919      PubMed Id: 27420616
Year: 2016  Volume: 62  Issue: 3  Pages: 259-264
Ability of fractions of cobra’s (Naja oxiana Eichwald) and copperhead snake’s (Agkistrodon halys halys) venoms to hydrolyze fibrinogen/fibrin was studied. In cobra’s snake a component with molecular mass of nearly 60 kDa was found to hydrolyze a-chain of fibrinogen but failed to hydrolyze casein/azocasein and fibrin. A fibrinogen-specific metalloproteinase, the enzyme was inhibited by EDTA. Cobra’s venom reduced the mass of donor’s fresh blood clots. The copperhead snake’s venom and the fractions obtained by gel-filtration (HW-50) and ion exchange chromatography (DEAE-650) were found to hydrolyze casein/azocasein, a- and b-chains of fibrinogen/fibrin and donor’s blood clots. The results from the study of the venom and proteolytically active fractions are the evidence for a thrombolytic potential in a copperhead snake’s venom.
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Yunusova E.S., Sadykov E.S., Sultanalieva N.M., Shkinev A.V. (2016) Biomeditsinskaya khimiya, 62(3), 259-264.
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