In MCF-7/Dox human breast carcinoma cells, down-regulation of integrin alpha5beta1 and inhibition of epidermal growth factor receptor (EGFR) markedly reduced rates of cell proliferation. Mitotic cycle analysis showed that alpha5beta1 down-regulation resulted in cell cycle arrest at the S phase, followed by a significant increase in the population of apoptotic cells (subG1 population). Inhibition of EGFR activity also caused cell cycle arrest at the S-phase but without any increase in the subG1 population. Down-regulation of alpha5beta1 and EGFR inhibition resulted in a significant decrease of cell content of the active (phosphorylated) forms of FAK and Erk protein kinases. The data obtained suggest that alpha5beta1 integrin is implicated in cell growth control via inhibition of apoptotic cell death and through EGFR activation.
Kozlova N.I., Morozevich G.E., Ushakova N.A., Gevorkian N.M., Berman A.E. (2016) Implication of integrin alpha5beta1 signal pathways in proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells. Biomeditsinskaya Khimiya, 62(3), 272-278.
Kozlova N.I. et al. Implication of integrin alpha5beta1 signal pathways in proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells // Biomeditsinskaya Khimiya. - 2016. - V. 62. -N 3. - P. 272-278.
Kozlova N.I. et al., "Implication of integrin alpha5beta1 signal pathways in proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells." Biomeditsinskaya Khimiya 62.3 (2016): 272-278.
Kozlova, N. I., Morozevich, G. E., Ushakova, N. A., Gevorkian, N. M., Berman, A. E. (2016). Implication of integrin alpha5beta1 signal pathways in proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells. Biomeditsinskaya Khimiya, 62(3), 272-278.
Morozevich G.E., Kozlova N.I., Ushakova N.A., Preobrazhenskaya M.E., Berman A.E., Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance, Biomeditsinskaya khimiya, 2011, vol: 57(1), 77-84. CrossRef Scholar google search
