Combined action of binase and bleomycin toward human lung adenocarcinoma cells

Zelenikhin P.V.1 , Makeeva A.V.1, Nguen T.N.1, Siraj Y.A.2, Ilinskaya O.N.1

1. Kazan (Volga Region) Federal University, Kazan, Russia
2. Kazan (Volga Region) Federal University, Kazan, Russia; College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia
Section: Experimental Study
DOI: 10.18097/PBMC20166203279      PubMed Id: 27420619
Year: 2016  Volume: 62  Issue: 3  Pages: 279-282
Some microbial ribonucleases (RNases) demonstrate selective cytotoxic effect against a wide range of tumor cells. In this context combined use of cytotoxic RNases in complex therapy with other chemotherapeutic agents appears to be especially promising. In this study we have investigated the apoptosis-induced effect of Bacillus pumilus RNase (binase) in combination with known anti-tumor antibiotic bleomycin on human lung adenocarcinoma A549 cells. The combined effect of high concentrations of these agents did not have any mutual increase in their apoptosis-induced action, while a combination of non-apoptotic concentrations resulted in the increase of the proportion of apoptotic cells up to 22% as compared with individual effect of bleomycin (6%) and binase (12%) used separately. These results indicate that binase and bleomycin are effective in combination of their low concentrations and ineffective in combination of their high concentrations.
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Keywords: cytotoxic ribonucleases, Bacillus pumilus, binase, bleomycin, antitumor activity, lung adenocarcinoma

Zelenikhin, P. V., Makeeva, A. V., Nguen, T. N., Siraj, Y. A., Ilinskaya, O. N. (2016). Combined action of binase and bleomycin toward human lung adenocarcinoma cells. Biomeditsinskaya Khimiya, 62(3), 279-282.
This paper is also available as the English translation: 10.1134/S1990750816010121
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