1. Institute of Biomedical Chemistry, Moscow, Russia 2. Foundation of Perspective Technologies and Innovations, Moscow, Russia 3. RES LTD. Moscow, Russia 4. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnjlogy (FSGEI of HPE), Moscow, Russia 5. Joint Institute for High Temperatures of RAS, Moscow, Russia
A combination of (atomic force microscopy)-based fishing (AFM-fishing) and mass spectrometry allows to capture protein molecules from solutions, concentrate and visualize them on an atomically flat surface of the AFM chip and identify by subsequent mass spectrometric analysis. In order to increase the AFM-fishing efficiency we have applied pulsed voltage with the rise time of the front of about 1 ns to the AFM chip. The AFM-chip was made using a conductive material, highly oriented pyrolytic graphite (HOPG). The increased efficiency of AFM-fishing has been demonstrated using detection of cytochrome b5 protein. Selection of the stimulating pulse with a rise time of 1 ns, corresponding to the GHz frequency range, by the effect of intrinsic emission from water observed in this frequency range during water injection into the cell.
Download PDF:
Keywords: ACM, MC, detection of low-copied proteins, protein fishing
Ivanov Yu.D. et al. AFM fishing of proteins under impulse electric field // Biomeditsinskaya Khimiya. - 2016. - V. 62. -N 4. - P. 439-446.
Ivanov Yu.D. et al., "AFM fishing of proteins under impulse electric field." Biomeditsinskaya Khimiya 62.4 (2016): 439-446.
Ivanov, Yu. D., Pleshakova, T. O., Malsagova, K. A., Kaysheva, A. L., Kopylov, A. T., Izotov, A. A., Tatur, V. Yu., Vesnin, S. G., Ivanova, N. D., Ziborov, V. S., Archakov, A. I. (2016). AFM fishing of proteins under impulse electric field. Biomeditsinskaya Khimiya, 62(4), 439-446.
Rissin D., Kan C., Campbell T., Howes S., Fournier D., Song L., Piech T., Patel P., Chang L., Rivnak A., Ferrel E., Randall J., Provuncher G., Walt D., Duffy D. (2010) Nature Biotech., 28, 595-599. CrossRef Scholar google search
Ivanov Y., Bukharina N., Pleshakova T., Frantsuzov P., Krokhin N., Ziborov V., Archakov A. (2011) J. Biophys., 56, 892-896. CrossRef Scholar google search
Ivanov Y., Pleshakova T., Malsagova K., Kozlov A., Kaysheva A., Kopylov A., Izotov A., Andreeva E.Y., Kanashenko S., Usanov S., Archakov A. (2014) FEBS J., 281, 4705-4717. Scholar google search
Haas P., Then P., Wild A., Grange W., Zorman S., Hegner M., Calame M., Aebi U., Flammer J., Hecht B. (2010) Anal. Chem., 82, 6299-6302. CrossRef Scholar google search
Pershin S., Bunkin A., Luk'yanchenko V.A. (2009) V Mezhdunarodnyj kongress “Slabye i sverkhslabye polya i izlucheniya v biologii i meditsine”, ss. 87-97. IBSN: 5-86456-007-3 Scholar google search
