1. Institute of Experimental Medicine, Saint Petersburg, Russia 2. Institute of Experimental Medicine, Saint Petersburg, Russia; Saint-Petersburg State University, Saint Petersburg, Russia
The study included 79 patients with coronary artery disease, 25 individuals with preclinical atherosclerosis and 59 healthy controls. Key lipid parameters were examined in all the participants. Levels of antibodies (Abs) against (IgG and IgM) LDL modified by malondialdehyde (MDA), acetic anhydride and hypochlorite, were determined by the enzyme-linked immunosorbent assay (ELISA). Abs specificity was tested by competitive ELISA. Circulating immune complexes (CIC) were isolated by precipitation in polyethylene glycol. Abs to hypochlorite-modified low density lipoprotein (hypochlorite-LDL) were detected in the serum samples. These Abs did not demonstrate cross-reactivity with MDA-modified LDL (MDA-LDL) and acetylated LDL (acetyl-LDL). Patients with coronary artery disease had increased levels of CIC (p<0.0001) and decreased levels of Abs (IgM) to hypochlorite-LDL, compared with healthy controls and patients with preclinical atherosclerosis (p=0.006). A correlation between the levels of Abs (IgG) to the hypochlorite-LDL and Abs to MDA- and acetyl-LDL was found. There was a correlation between the content of the Abs (IgM) to MDA- and acetyl-LDL and the concentration of CIC-cholesterol. Lipid parameters did not correlate with Abs levels.
Belik I.V., Ivantsova A.A., Mamedova Z.E., Denisenko A.D. (2016) Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis. Biomeditsinskaya Khimiya, 62(4), 471-475.
Belik I.V. et al. Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis // Biomeditsinskaya Khimiya. - 2016. - V. 62. -N 4. - P. 471-475.
Belik I.V. et al., "Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis." Biomeditsinskaya Khimiya 62.4 (2016): 471-475.
Belik, I. V., Ivantsova, A. A., Mamedova, Z. E., Denisenko, A. D. (2016). Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis. Biomeditsinskaya Khimiya, 62(4), 471-475.
Resch U., Semlitsch M., Hammer A., Susani-Etzerodt H., Walczak H., Sattler W., Malle E. (2011) Biochem. Biophys. Res. Commun., 410, 895-900. CrossRef Scholar google search
Salonen J.T., Ylä-Herttuala S., Yamamoto R., Butler S., Korpela H., Salonen R., Nyyssönen K., Palinski W., Witztum J.L. (1992) Lancet., 339(8798), 883-887. CrossRef Scholar google search
Virella G.I., Virella I., Leman R.B., Pryor M.B., Lopes-Virella M.F. (1993) Int. J. Clin. Lab. Res., 23(2), 95-101. CrossRef Scholar google search
Tinahones F.J., Gómez-Zumaquero J.M., Garrido-Sánchez L., García-Fuentes E., Rojo-Martínez G., Esteva I., Ruiz de Adana M.S., Cardona F., Soriguer F. (2005) J. Lipid Res., 46, 452-457. CrossRef Scholar google search
Virella G., Atchley D., Koskinen S., Zheng D., Lopes-Virella M.F.; DCCT/EDIC Research Group (2002) Clin. Immunol., 105(1), 81-92. CrossRef Scholar google search
Malle E., Hazell L., Stocker R., Sattler W., Esterbauer H., Waeg G. (1995) Arterioscler. Thromb. Vasc. Biol., 15, 982-989. CrossRef Scholar google search
