The hepatic differentiation of adult and fetal liver stromal cells in vitro

Kholodenko I.V.1 , Kholodenko R.V.2, Manukyan G.V.3, Yarygin K.N.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
3. Russian National Research Center of Surgery, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20166206674      PubMed Id: 28026812
Year: 2016  Volume: 62  Issue: 6  Pages: 674-682
The liver has a marked capacity for regeneration. In most cases the liver regeneration is determined by hepatocytes. The regenerative capacity of hepatocytes is significantly reduced in acute or chronic damage. In particular, repair mechanisms are not activated in patients with alcoholic cirrhosis. Organ transplantation or advanced methods of regenerative medicine can help such patients. The promising results were obtained in clinical trials involving patients with various forms of liver disease who received transplantation of autologous bone marrow stem cells. However, to improve the effectiveness of such treatment it is necessary to search for more optimal sources of progenitor cells, as well as to evaluate the possibility of using descendants of these cells differentiated in vitro. In this study we isolated stromal cells from the liver biopsies of three patients with alcoholic cirrhosis, conducted their morphological and phenotypic analysis, and evaluated the hepatic potential of these cells in vitro. The stromal cells isolated from fetal liver were used for comparison. The results of this can serve as a basis for the development of a new method for the treatment of end-stage liver disease. The stromal cells isolated from the liver biopsies for a long time proliferate in a culture and this which makes it possible to expand them to large amounts for subsequent differentiation into hepatocyte-like cells and autologous transplantation.
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Keywords: adult liver stromal cells, fetal liver stromal cells, hepatic differentiation, alcoholic cirrhosis, hepatocyte-like cells

Kholodenko, I. V., Kholodenko, R. V., Manukyan, G. V., Yarygin, K. N. (2016). The hepatic differentiation of adult and fetal liver stromal cells in vitro. Biomeditsinskaya Khimiya, 62(6), 674-682.
This paper is also available as the English translation: 10.1134/S1990750817030052
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