1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia 2. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia 3. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia
Perioperative corneal abrasion is an ophthalmic complication commonly found in patients underwent general anesthesia. In this study, correlations between development of corneal injury and proteomic changes in tear film during general anesthesia were examined using an animal (rabbit) model. Being started after 1-h anesthesia, the process of accumulation of pathological changes in the cornea unequivocally led clinically significant abrasions following 3-6 h of the narcosis. The corneal damage was associated with alterations in profiles of major proteins of the tear film. Analysis of the tear proteome pointed to depression of lachrymal glands function, and suggested serotransferrin, serum albumin and annexin A1 as potential tear markers of the complication. The tear film alterations included fast drop of total antioxidant activity and activity of superoxide dismutase, and decrease in interleukin-4 and increase in interleukin-6 content indicating development of oxidative and pro-inflammatory responses. These findings suggest antioxidant and anti-inflammatory therapy as prospective approach for prevention/treatment of perioperative corneal abrasions. The observed anesthesia-induced effects should be considered in any study of ocular surface diseases employing anesthetized animals.
National Research Council (U.S.). Committee for the Update of the Guide for the Care and Use of Laboratory Animals., Institute for Laboratory Animal Research (U.S.), and National Academies Press (U.S.). (2011) Guide for the care and use of laboratory animals. 8th Ed., National Academies Press,, Washington, D.C. Scholar google search
(2016)Association for Research in Vision and Ophthalmology. Statement for the Use of Animals in Ophthalmic and Visual Research. http://www.arvo.org/about_arvo/policies/statement_ for_the_use_of_animals_in_ophthalmic_and_visual_research/. Scholar google search
Samra Z., Lazarovich T., Sompolinsky D. (1989) J. Clin. Chem. Clin. Biochem., 27, 505-509. Scholar google search
Wakamatsu T.H., Dogru M., Matsumoto Y., Kojima T., Kaido M., Ibrahim O.M., Sato E.A., Igarashi A., Ichihashi Y., Satake Y., Shimazaki J., Tsubota K. (2013) Invest. Ophthalmol. Vis. Sci., 54, 201-210. CrossRef Scholar google search