GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value
1. Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia 2. Moscow Institute of Physics and Technology, Dolgoprudniy, Moscow Region, Russia 3. Moscow State Medical Stomatological University, Moscow, Russia 4. Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudniy, Moscow Region, Russia
There is a clear need in molecular markers for prostate cancer (PC) risk stratification. Alteration of DNA methylation is one of processes that occur during ÐÑ progression. Methylation-sensitive PCR with high resolution melting curve analysis (MS-HRM) can be used for gene methylation analysis in routine laboratory practice. This method requires very small amounts of DNA for analysis. Numerous results have been accumulated on DNA methylation in PC samples analyzed by the Infinium HumanMethylation450 BeadChip (HM450). However, the consistency of MS-HRM results with chip hybridization results has not been examined yet. The aim of this study was to assess the consistency of results of GSTP1, APC and RASSF1 gene methylation analysis in ÐÑ biopsy samples obtained by MS-HRM and chip hybridization. The methylation levels of each gene determined by MS-HRM were statistically different in the group of PC tissue samples and the samples without signs of tumor growth. Chip hybridization data analysis confirmed the results obtained with the MS-HRM. Differences in methylation levels between tumor tissue and histologically intact tissue of each sample determined by MS-HRM and chip hybridization, were consistent with each other. Thus, we showed that the assessment of GSTP1, APC and RASSF1 gene methylation analysis using MS-HRM is suitable for the design of laboratory assays that will differentiate the PC tissue from the tissue without signs of tumor growth.
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Keywords: prostate cancer, DNA methylation, HRM, GSTP1, APC, RASSF1
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Skorodumova L.O., Babalyan K.A., Sultanov R., Vasiliev A.O., Govorov A.V., Pushkar D.Y., Prilepskaya E.A., Danilenko S.A., Generozov E.V., Larin A.K., Kostryukova E.S., Sharova E.I. (2016) GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value. Biomeditsinskaya Khimiya, 62(6), 708-714.
Skorodumova L.O. et al. GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value // Biomeditsinskaya Khimiya. - 2016. - V. 62. -N 6. - P. 708-714.
Skorodumova L.O. et al., "GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value." Biomeditsinskaya Khimiya 62.6 (2016): 708-714.
Skorodumova, L. O., Babalyan, K. A., Sultanov, R., Vasiliev, A. O., Govorov, A. V., Pushkar, D. Y., Prilepskaya, E. A., Danilenko, S. A., Generozov, E. V., Larin, A. K., Kostryukova, E. S., Sharova, E. I. (2016). GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value. Biomeditsinskaya Khimiya, 62(6), 708-714.
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