The effect of neuroprotector isatin on binding of some model proteins with beta-amyloid peptide: a biosensor study

Buneeva O.A.1, Gnedenko O.V.1, Medvedeva M.V.2, Ivanov A.S.1, Medvedev A.E.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Moscow State University, Moscow, Russia
Section: Short Communication
DOI: 10.18097/PBMC20166206720      PubMed Id: 28026818
Year: 2016  Volume: 62  Issue: 6  Pages: 720-724
The amyloid-beta peptide 1-42 formed during proteolytic processing of the amyloid precursor protein (APP) plays a key role in the development or progression of Alzheimer's disease (AD) and other pathologies associated with formation of protein aggregates in the central nervous system. Recent proteomic profiling of mouse and rat brain preparations by means of beta-amyloid peptide immobilized on Affigel-10 revealed a large group of amyloid-binding proteins (n>80). Many (about 25%) of these proteins were previously identified as isatin-binding proteins. The aim of this study was to validate direct interaction between beta-amyloid peptide and highly purified intact and oxidized peroxiredoxin, M-type pyruvate kinase, alpha-enolase, and the effect of isatin on this interaction. The study performed using SPR-based Biacore 3000 and Biacore X100 biosensors has shown that all the proteins form molecular complexes with immobilized beta-amyloid peptide. The Kd values for these complexes varied from 8.36х10-8 M (peroxiredoxin) to 1.97х10-6 M (alpha-enolase). Oxidative modification of investigated proteins caused opposite effects on complexes of these peptides with beta-amyloid. The endogenous neuroprotector isatin increased dissociation of complexes formed by beta-amyloid peptide with both intact proteins (peroxiredoxin, glyceraldehyde-3-phosphate dehydrogenase) and/or oxidized proteins (peroxiredoxin, pyruvate kinase) used in this study.
Download PDF:  
Keywords: beta-amyloid peptide, amyloid-beta binding proteins, isatin, ligand-receptor and protein-protein interactions, surface plasmon resonance, optical biosensor

Buneeva, O. A., Gnedenko, O. V., Medvedeva, M. V., Ivanov, A. S., Medvedev, A. E. (2016). The effect of neuroprotector isatin on binding of some model proteins with beta-amyloid peptide: a biosensor study. Biomeditsinskaya Khimiya, 62(6), 720-724.
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)