Comparison of the expression profile of surface molecular markers on mesenchymal stromal cell
cultures isolated from human endometrium and umbilical cord
Endometrial mesenchymal stromal cells (eMSCs), along with mesenchymal stromal cells (MSCs) isolated from other tissues, are promising for use in regenerative medicine. The benefits of eMSCs include their presence in adults, simplicity of isolation, high proliferative and differentiation capacity. In this study, we have employed the flow cytometry technique to assess expression of 28 molecular markers on the surface of two eMSCs cultures. The culture of MSCs isolated from Wharton's jelly of the umbilical cord (uMSCs) was used as a reference, because uMSCs were studied in details earlier and demonstrated their effectiveness in vivo. Both types of MSCs demonstrated similar expression profiles. They included stem cells surface molecules, cell adhesion molecules and their ligands, some receptor molecules responsible for cell metabolism and proliferation, as well as immunological response molecules.
Lupatov A.Yu., Saryglar R.Yu., Chuprynin V.D., Pavlovich S.V., Yarygin K.N. (2017) Comparison of the expression profile of surface molecular markers on mesenchymal stromal cell
cultures isolated from human endometrium and umbilical cord. Biomeditsinskaya Khimiya, 63(1), 85-90.
Lupatov A.Yu. et al. Comparison of the expression profile of surface molecular markers on mesenchymal stromal cell
cultures isolated from human endometrium and umbilical cord // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 1. - P. 85-90.
Lupatov A.Yu. et al., "Comparison of the expression profile of surface molecular markers on mesenchymal stromal cell
cultures isolated from human endometrium and umbilical cord." Biomeditsinskaya Khimiya 63.1 (2017): 85-90.
Lupatov, A. Yu., Saryglar, R. Yu., Chuprynin, V. D., Pavlovich, S. V., Yarygin, K. N. (2017). Comparison of the expression profile of surface molecular markers on mesenchymal stromal cell
cultures isolated from human endometrium and umbilical cord. Biomeditsinskaya Khimiya, 63(1), 85-90.
Can A., Ulus A.T., Cinar O., Topal Celikkan F., Simsek E., Akyol M., Canpolat U., Erturk M., Kara F., Ilhan O. (2015) Stem Cell Rev., 11, 752-760. CrossRef Scholar google search
Zhang Z., Lin H., Shi M., Xu R., Fu J., Lv J., Chen L., Lu S., Li Y., Yu S., Geng H., Jin L., Lau G.K., Wang F.S. (2012) J. Gastroenterol. Hepatol., 27(Suppl. 2), 112-120. CrossRef Scholar google search
Li J.F., Zhang D.J., Geng T., Chen L., Huang H., Yin H.L., Zhang Y.Z., Lou J.Y., Cao B., Wang Y.L. (2014) Cell Transplant., 23(Suppl. 1), 113-122. CrossRef Scholar google search
Dominici M., Le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F., Krause D., Deans R., Keating A., Prockop Dj., Horwitz E. (2006) Cytotherapy, 8(4), 315-317. CrossRef Scholar google search
Moretti P., Hatlapatka T., Marten D., Lavrentieva A., Majore I., Hass R., Kasper C. (2010) Adv. Biochem. Eng. Biotechnol., 123, 29-54. Scholar google search
