Using poly(lactic-co-glycolic) acid we developed a polymeric form of niclosamide (PFN) and investigated molecular mechanisms underlying its antitumor activity against human colorectal cancer cell lines (SW837, Caco-2, COLO 320 HSR). PFN was shown to be more cytotoxic against cancer cells and less cytotoxic against normal cells (human embryonic lung fibroblasts) as compared to niclosamide. Both niclosamide and its polymeric form caused mitochondrial damage (evaluated as a decrease in rhodamine 123 accumulation) and increased the levels of reactive oxygen species, particularly mitochondrial superoxide, resulting in the oxidative damage to biomolecules. Furthermore, niclosamide and PFN induced G0/G1 cell cycle arrest.
Download PDF:
Keywords: niclosamide, polymeric form of niclosamide, poly(lactic-co-glycolic) acid, reactive oxygen species, cell cycle, colorectal cancer
Citation:
Zhirnik A.S., Semochkina Y.P., Moskaleva E.Yu., Krylov N.I., Tubasheva I.A., Kuznetsov S.L., Vorontsov E.A. (2017) Antineoplastic mechanisms of niclosamide-loaded nanoparticles in human colorectal cancer cells. Biomeditsinskaya Khimiya, 63(2), 132-138.
Zhirnik A.S. et al. Antineoplastic mechanisms of niclosamide-loaded nanoparticles in human colorectal cancer cells // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 2. - P. 132-138.
Zhirnik A.S. et al., "Antineoplastic mechanisms of niclosamide-loaded nanoparticles in human colorectal cancer cells." Biomeditsinskaya Khimiya 63.2 (2017): 132-138.
Zhirnik, A. S., Semochkina, Y. P., Moskaleva, E. Yu., Krylov, N. I., Tubasheva, I. A., Kuznetsov, S. L., Vorontsov, E. A. (2017). Antineoplastic mechanisms of niclosamide-loaded nanoparticles in human colorectal cancer cells. Biomeditsinskaya Khimiya, 63(2), 132-138.
Ostadhossein F., Misra S.K., Mukherjee P., Ostadhossein A., Daza E., Tiwari S., Mittal S., Gryka M.C., Bhargava R., Pan D. (2016) Small. CrossRef Scholar google search
Russo A., Pellosi D.S., Pagliara V., Milone M.R., Pucci B., Caetano W., Hioka N., Budillon A., Ungaro F., Russo G., Quaglia F. (2016) Int. J. Pharm., 511(1), 127-139. CrossRef Scholar google search
Kuznetsova I.G., Severin S.E. (2013) Razrabotka i registratsiya lekarstvennykh sredstv, 5(5), 30-36. Scholar google search
Lu J.M., Wang X., Marin-Muller C., Wang H., Lin P.H., Yao Q., Chen C. (2009) Expert Rev. Mol. Diagn., 9(4), 325-341. CrossRef Scholar google search
