PCR analysis of the absolute number of copies of human chromosome 18 transcripts in liver and HepG2 cells

Kiseleva Y.Y.1, Ptitsyn K.G.1, Tikhonova O.V.1, Radko S.P.1 , Kurbatov LK.1, Vakhrushev I.V.1, Zgoda V.G.1, Ponomarenko E.A.1, Lisitsa A.V.1, Archakov A.I.1

1. Institute of Biomedical Chemistry, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20176302147      PubMed Id: 28414286
Year: 2017  Volume: 63  Issue: 2  Pages: 147-153
Using reverse transcription in conjunction with the quantitative real-time PCR or digital droplet PCR, the transcriptome profiling of human chromosome 18 has been carried out in liver hepatocytes and hepatoblastoma cells (HepG2 cell line) in terms of the absolute number of each transcript per cell. The transcript abundance varies within the range of 0.006 to 9635 and 0.011 to 4819 copies per cell for HepG2 cell line and hepatocytes, respectively. The expression profiles for genes of chromosome 18 in hepatocytes and HepG2 cells were found to significantly correlate: the Spearman's correlation coefficient was equal to 0.81. The distribution of frequency of transcripts over their abundance was bimodal for HepG2 cells and unimodal for liver hepatocytes. Bioinformatic analysis of the differential gene expression has revealed that genes of chromosome 18, overexpressed in HepG2 cells compared to hepatocytes, are associated with cell division and cell adhesion processes. It is assumed that the enhanced expression of those genes in HepG2 cells is related to the proliferation activity of cultured cells. The differences in transcriptome profiles have to be taken into account when modelling liver hepatocytes with cultured HepG2 cells.
Download PDF:  
Keywords: transcriptome, quantitative PCR, chromosome 18, hepatocytes, HepG2

Kiseleva, Y. Y., Ptitsyn, K. G., Tikhonova, O. V., Radko, S. P., Kurbatov, LK., Vakhrushev, I. V., Zgoda, V. G., Ponomarenko, E. A., Lisitsa, A. V., Archakov, A. I. (2017). PCR analysis of the absolute number of copies of human chromosome 18 transcripts in liver and HepG2 cells. Biomeditsinskaya Khimiya, 63(2), 147-153.
This paper is also available as the English translation: 10.1134/S1990750818010067
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)