1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia 2. Tomsk Cancer Research Institute, Tomsk, Russia 3. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia 4. Novosibirsk Regional Oncological Dispensary, Novosibirsk, Russia
A simple approach for isolation of exosomes from the blood plasma, which allows to obtain highly purified preparations of microvesicles no larger than 100 nm has been proposed. The presence of different subpopulations of exosomes in the blood plasma of healthy donors and cancer patients has been recognized. We found the presence of the universal markers CD9, CD24 and CD81 on exosomes isolated from blood plasma that can be used to their routine typing.
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Keywords: exosomes, transmission electron microscopy, flow cytometry, colorectal cancer, breast cancer
Citation:
Tamkovich S.N., Yunusova N.V., Stakheeva M.N., Somov A.K., Frolova A.Y., Kirushina N.A., Afanasyev S.G., Grigoryeva A.E., Laktionov P.P., Kondakova I.V. (2017) Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. Biomeditsinskaya Khimiya, 63(2), 165-169.
Tamkovich S.N. et al. Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 2. - P. 165-169.
Tamkovich S.N. et al., "Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients." Biomeditsinskaya Khimiya 63.2 (2017): 165-169.
Tamkovich, S. N., Yunusova, N. V., Stakheeva, M. N., Somov, A. K., Frolova, A. Y., Kirushina, N. A., Afanasyev, S. G., Grigoryeva, A. E., Laktionov, P. P., Kondakova, I. V. (2017). Isolation and characterization of exosomes from blood plasma of breast cancer and colorectal cancer patients. Biomeditsinskaya Khimiya, 63(2), 165-169.
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