Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism (monoaminbe oxidases A and B (MAO A and MAO B) and catechol-O-methyl transferase (COMT)) and renalase has been carried out in tissues of normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Among investigated tissues the highest level of mRNA of genes encoding key enzymes of catecholamine catabolism (MAO A, MAO B, COMT) was found in the heart of WKY rats. In SHR the mRNA levels of these genes were lower (p<0.05-0.01), however, no similar changes were observed in the tissues studied in dependence of hypertension. The relative mRNA levels of the studied genes normalized versus actin mRNA significantly varied. In heart and kidney the relative level of COMT mRNA significantly exceeded the relative levels of both MAO A mRNA and MAO B mRNA. In the brain differences in mRNAs of MAOA, MAOB, and COMT were less pronounced. However, in all examined tissue the renalase mRNA level was much (at least 10-20-fold) lower than any other mRNA studied. Taking into consideration known correlations between mRNAs and corresponding protein products reported in the literature for many genes these results suggest that in the case of any catalytic scenarios proposed or even proved for renalase this protein cannot contribute to catecholamine degradation. It is also unlikely that the products of renalase reaction, b-NAD(P)+ and hydrogen peroxide, can exhibit a hypotensive effect due to low expression of the renalase encoding gene.
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Keywords: renalase, gene expression, genes encoding monoamine oxidases and catechol-O-methyl transferase, SHR and WKY rats
Citation:
Fedchenko V.I., Medvedev A.E. (2017) Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism and renalase in tissues of normotensive and hypertensive rats. Biomeditsinskaya Khimiya, 63(4), 312-315.
Fedchenko V.I. et al. Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism and renalase in tissues of normotensive and hypertensive rats // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 4. - P. 312-315.
Fedchenko V.I. et al., "Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism and renalase in tissues of normotensive and hypertensive rats." Biomeditsinskaya Khimiya 63.4 (2017): 312-315.
Fedchenko, V. I., Medvedev, A. E. (2017). Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism and renalase in tissues of normotensive and hypertensive rats. Biomeditsinskaya Khimiya, 63(4), 312-315.
Xu J., Li G., Wang P., Velazquez H., Yao X., Li Y., Wu Y., Peixoto A., Crowley S., Desir G.V. (2005) J. Clin. Invest., 115, 1275-1280. CrossRef Scholar google search
Zhao Q., Fan Z., He J., Chen S., Li H., Zhang P., Wang L., Hu D., Huang J., Qiang B., Gu D. (2007) J. Mol. Med., 85, 877-885. CrossRef Scholar google search
Ghosh S.S., Krieg R.J., Sica D.A., Wang R., Fakhry I., Gehr T. (2009) Pediatr. Nephrol., 24, 367-377. CrossRef Scholar google search
Fedchenko V., Kopylov A., Kozlova N., Buneeva O., Kaloshin A., Zgoda V., Medvedev A. (2016) Kidney Blood Pressure Research, 41, 593-603. CrossRef Scholar google search
Fedchenko V., Globa A., Buneeva O., Medvedev A. (2013) Medical Science Monitor Basic Research, 19, 267-270. CrossRef Scholar google search
Fedchenko V., Globa A., Kaloshin A., Kapitsa I., Nerobkova L., Val’dman E., Buneeva O., Glover V., Medvedev A. (2008) Medical Science Monitor, 14, BR269–BR273. Scholar google search
